Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
Vet J. 2012 Jun;192(3):547-9. doi: 10.1016/j.tvjl.2011.08.025. Epub 2011 Sep 25.
Ovar-DRB1 is one of the most important response genes in the major histocompatibility complex (MHC) class II region of sheep. Gene polymorphism in the second exon of Ovar-DRB1 in three different Iranian fat-tailed sheep breeds (Lori-Bakhtiari, Shaul and Zandi) was analyzed by either restriction fragment length polymorphisms (RFLP) or direct sequencing. A total of 92 Lori-Bakhtiari, 40 Shaul, and 47 Zandi sheep were examined. PCR-RFLP identified 17 genotypes in Lori-Bakhtiari sheep, 12 in Shaul sheep and 11 in Zandi sheep. Collectively, 24 different genotypes could be found for Iranian fat-tailed sheep. Using direct sequencing, seven new sequences in exon 2 of the Ovar-DRB1 gene were identified. Generalized linear modeling with a multinomial error structure showed that the sheep populations had significantly different allele frequencies.
卵巢 DRB1 是绵羊主要组织相容性复合体（MHC）Ⅱ类区域中最重要的反应基因之一。通过限制性片段长度多态性（RFLP）或直接测序分析了三种不同伊朗肥尾绵羊品种（Lori-Bakhtiari、Shaul 和 Zandi）的 Ovar-DRB1 第二外显子的基因多态性。共检查了 92 只 Lori-Bakhtiari、40 只 Shaul 和 47 只 Zandi 绵羊。PCR-RFLP 在 Lori-Bakhtiari 绵羊中鉴定出 17 种基因型，在 Shaul 绵羊中鉴定出 12 种，在 Zandi 绵羊中鉴定出 11 种。伊朗肥尾绵羊共发现 24 种不同的基因型。通过直接测序，在 Ovar-DRB1 基因的外显子 2 中鉴定出 7 个新序列。具有多项错误结构的广义线性建模表明，绵羊群体的等位基因频率存在显著差异。
