Gruszczyńska Joanna, Brokowska Karolina, Charon Krystyna M, Swiderek Wiesław P
Department of Genetics and Animal Breeding, Warsaw Agricultural University, Ciszewskiego 8, 02-786 Warsaw, Poland.
J Appl Genet. 2005;46(3):311-4.
Exon 2 of the Ovar-DR gene is known to encode the MHC outer domain (alpha or beta chain) that forms the binding area to antigens presented. The study was aimed at analysing exon 2 Ovar -DRB1 gene polymorphism in Polish Heath Sheep and Polish Lowland Sheep (Zelazna variety). A total of 101 and 99 ewes of the respective breeds were included in this study. We identified 65 different haplotypes in Polish Heath Sheep and 68 in Polish Lowland Sheep. The PCR-RFLP method and PCR products sequencing made it possible to identify two new sequences of exon 2 Ovar-DRB1 gene (AY230000 and AY248695). A distinct polymorphism in the exon 2 sequence presents possibilities for immune response toward a great variety of pathogens.
已知卵巢 - DR基因的外显子2编码主要组织相容性复合体（MHC）的外部结构域（α或β链），该结构域形成与呈递抗原的结合区域。本研究旨在分析波兰 Heath 羊和波兰低地羊（Zelazna品种）的外显子2卵巢 - DRB1基因多态性。本研究分别纳入了101只和99只各品种的母羊。我们在波兰 Heath 羊中鉴定出65种不同的单倍型，在波兰低地羊中鉴定出68种。PCR-RFLP方法和PCR产物测序使得鉴定卵巢 - DRB1基因外显子2的两个新序列（AY230000和AY248695）成为可能。外显子2序列中明显的多态性为针对多种病原体的免疫反应提供了可能性。
