Laboratorio de Micologia y Fitopatologia, Universidad de Los Andes, Colombia.
Toxicon. 2011 Nov;58(6-7):583-90. doi: 10.1016/j.toxicon.2011.09.005. Epub 2011 Sep 16.
Some species in the genus Amanita have a great variety of toxic secondary metabolites. They are characterized macroscopically by having a white spore print and free gills, and microscopically by the presence of a divergent hymenophoral trama. Some species of Amanita present in Colombia were chemically characterized by analyzing their toxin composition using HPLC. Samples were collected in oak (Quercus humboldtii) and pine (Pinus radiata) forests. Twelve species were recovered, Amanita fuligineodisca, Amanita xylinivolva, Amanita flavoconia, Amanita rubescens, Amanita bisporigera, Amanita muscaria, Amanita humboldtii, Amanita sororcula, Amanita brunneolocularis, Amanita colombiana, Amanita citrina, Amanita porphyria as well as two unreported species. Results showed that most of the analyzed species have α -amanitin in concentrations ranging from 50 ppm to 6000 ppm. Concentrations of α-amanitin in the pileus were significantly greater than in the stipe. Phalloidin and phallacidin were only present in A. bisporigera. Chromatographic profiles are proposed as an additional taxonomic tool since specific peaks with similar retention times were conserved at the species level.
鹅膏属的一些物种具有丰富多样的有毒次生代谢物。它们在宏观上的特征是具有白色的孢子印痕和自由的菌褶，在微观上的特征是发散的菌褶组织。使用高效液相色谱法分析毒素组成，对哥伦比亚存在的一些鹅膏属物种进行了化学特征分析。样本是在栎（Quercus humboldtii）和松（Pinus radiata）林中采集的。共回收了 12 种物种，包括灰鹅膏菌（Amanita fuligineodisca）、木生鹅膏菌（Amanita xylinivolva）、黄鹅膏菌（Amanita flavoconia）、红鹅膏菌（Amanita rubescens）、双孢鹅膏菌（Amanita bisporigera）、毒蝇鹅膏菌（Amanita muscaria）、亨氏鹅膏菌（Amanita humboldtii）、姊妹鹅膏菌（Amanita sororcula）、棕灰鹅膏菌（Amanita brunneolocularis）、哥伦比亚鹅膏菌（Amanita colombiana）、金黄鹅膏菌（Amanita citrina）、红托鹅膏菌（Amanita porphyria）以及两种未报告的物种。结果表明，大多数分析的物种的α-鹅膏菌素浓度在 50ppm 到 6000ppm 之间。菌盖中的α-鹅膏菌素浓度明显大于菌柄。在双孢鹅膏菌中仅存在鹅膏蕈肽和鹅膏蕈氨酸。色谱图谱被提议作为一种额外的分类工具，因为在种水平上保留了具有相似保留时间的特定峰。
