Institute of Molecular Biology, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria.
Department of Physical and Macromolecular Chemistry, Faculty of Science, Charles University, 128 00 Prague, Czech Republic.
Molecules. 2023 Sep 27;28(19):6824. doi: 10.3390/molecules28196824.
The fungus is universally recognizable for its iconic appearance; it is also widely regarded as poisonous, inedible, and even deadly. In spite of that, there have been documented cases of use of -containing preparations against various diseases, including cancer, to no apparent ill effect. The search for compounds that can be used to treat cancer among various plants and fungi has been intensifying in recent years. In light of this, we describe an HPLC HILIC analytical method for the evaluation of the content of the anticancer compound ergosterol (ERG) and the neuroactive alkaloids ibotenic acid (IBO) and muscimol (MUS) that contribute significantly to the unpleasant physiological syndrome associated with consumption. A 'homemade' tincture made using 80-proof rye vodka as the solvent, an extract made with a standardized water-ethanol solution as the solvent, and fractions obtained from the second extract via liquid-liquid extraction with nonpolar solvents were analyzed. The study also presents the results of capillary zone electrophoresis with contactless conductivity detection and UHPLC-MS/MS analyses of the IBO and MUS content of the two native extracts and an evaluation of the standardized extract's cytotoxic effect against a small panel of lung cell cultures in vitro. Our results show that the standardized extract has a significant cytotoxic effect and does not contain the compounds of interest in any significant quantity.
这种真菌因其标志性的外观而被普遍认可；它也被广泛认为是有毒的、不可食用的，甚至是致命的。尽管如此，仍有记录在案的使用含有 - 制剂治疗各种疾病的案例，包括癌症，而且没有明显的不良影响。近年来，人们一直在加紧从各种植物和真菌中寻找可用于治疗癌症的化合物。有鉴于此，我们描述了一种用于评估抗癌化合物麦角固醇（ERG）和神经活性生物碱（IBO）和（MUS）含量的 HPLC HILIC 分析方法，这些化合物对与食用相关的不愉快生理综合征有重要贡献。一种“自制”的酊剂，使用 80 度的黑麦伏特加作为溶剂，一种用标准化的水-乙醇溶液作为溶剂的提取物，以及通过用非极性溶剂进行液-液萃取从第二种提取物中获得的馏分进行了分析。该研究还介绍了毛细管区带电泳与接触式传导检测以及两种天然提取物中 IBO 和 MUS 含量的 UHPLC-MS/MS 分析结果，并评估了标准化提取物对体外小面板肺细胞培养物的细胞毒性作用。我们的结果表明，标准化提取物具有显著的细胞毒性作用，并且不以任何显著量含有感兴趣的化合物。
