Department of Toxicology, Medical University of Bialystok, Mickiewicza 2c, 15-222, Bialystok, Poland.
Bull Environ Contam Toxicol. 2011 Dec;87(6):638-42. doi: 10.1007/s00128-011-0400-2. Epub 2011 Sep 27.
In the present study we examined a role of pro-apoptotic Bax and anti-apoptotic Mcl-1 proteins, participating in the regulation of intrinsic apoptosis pathway in human neutrophils (PMNs) exposed to N-nitrosodimethylamine (NDMA), the environmental xenobiotic. For the purpose comparison, the same studies were conducted in autologous peripheral blood mononuclear cells (PBMCs). The production of cytochrome c by PMNs was also determined. A deficit of anti-apoptotic Mcl-1 and overexpression of the pro-apoptotic protein Bax suggest that the apoptosis process in human neutrophils exposed to NDMA is dependent on changes in the expression of these proteins. PMNs were more sensitive to NDMA than PBMCs.
在本研究中，我们研究了促凋亡 Bax 和抗凋亡 Mcl-1 蛋白在调节人类嗜中性粒细胞（PMN）中固有凋亡途径的作用，这些细胞暴露于环境异生物质 N-亚硝二甲胺（NDMA）。为了进行比较，我们还在同种异体外周血单核细胞（PBMC）中进行了相同的研究。还测定了 PMN 细胞色素 c 的产生。PMN 中抗凋亡 Mcl-1 的缺乏和促凋亡蛋白 Bax 的过表达表明，暴露于 NDMA 的人类嗜中性粒细胞中的凋亡过程依赖于这些蛋白表达的变化。PMN 比 PBMC 对 NDMA 更敏感。
