Xu S W
Second Hospital, Lanzhou Medical College.
Zhonghua Zhong Liu Za Zhi. 1990 Jan;12(1):30-3.
After the human gastric cancer cells SGC-7901 were cultured with 5 mM hexamethylene bisacetamide (HMBA) for 120 hr, cell growth was inhibited. The doubling time increased from 48 hr in untreated cells to 99.6 hr in HMBA-treated cells. The maximal increase in growth was 2.4-fold as compared to 6.4-fold in the untreated control. 3H-thymidine incorporation was inhibited by HMBA and the colony formation rate was reduced. There was concomitant decrease in carcino-embryonic antigen and beta-2 microglobulin as determined by radioimmuno-assay. Lactic dehydrogenase (LDH) and its isoenzyme assay revealed a marked increase in H-type LDH but the total enzyme activity was reduced. These results indicate that HMBA inhibits proliferation of SGC-7901 cells in vitro.
人胃癌细胞SGC - 7901用5 mM己二酰双乙酰胺（HMBA）培养120小时后，细胞生长受到抑制。倍增时间从未经处理细胞的48小时增加到HMBA处理细胞的99.6小时。与未处理对照的6.4倍相比，生长的最大增加倍数为2.4倍。HMBA抑制3H - 胸腺嘧啶核苷掺入，集落形成率降低。放射免疫测定法测定显示癌胚抗原和β2微球蛋白同时减少。乳酸脱氢酶（LDH）及其同工酶测定显示H型LDH显著增加，但总酶活性降低。这些结果表明HMBA在体外抑制SGC - 7901细胞的增殖。
