Lu Xiaoying, Su Miancheng, Li You, Zeng Linfu, Liu Xinghua, Li Jianming, Zheng Baochun, Wang Shuangyin
Institute of Military Medical Sciences, Chengdu Military Command, Kunming 650032, China.
Chin Med J (Engl). 2002 May;115(5):716-21.
To study the inhibitory effect of Acanthopanax giraldii Harms Var. Hispidus Hoo polysaccharides (AGP) on SGC-7901 gastric cancer cells and its possible mechanism.
Cell doubling time analysis, colony forming assay and MTT assay were adopted to study the inhibitory effect and its characteristics. We also analyzed the amount of protein expressed by oncogenes, antioncogenes and cell factors using flow cytometric analysis.
AGP inhibited the proliferation of SGC-7901 cells and cell colony forming ability. AGP did not inhibit the viability and function of lymphocytes of peripheral blood in healthy subjects and human embryonic tenocytes, except for the highest dosage of AGP (P < 0.05), which slightly inhibited the viability and function of the two types of normal cells. AGP inhibited the viability and function of SGC-7901 cells, except for the lowest dosages of AGP I and AGP III. There was a dose-effect relationship between the dosage of the AGP and SGC-7901 cells. The effect of the AGP at the molecular level was associated with the low protein expression of the c-myc and bcl-2 genes and the high protein expression of the p53, bax, fas and fas-L genes, as well as the cell factor TGF beta(1). The inhibitory effect of AGP was weaker than that of CDDP, but was stronger than that of Vitamin C.
Acanthopanax giraldii Harms Var. Hispidus Hoo polysaccharides selectively inhibited the proliferation, the colony forming ability, and the viability and function of human gastric cancer cells through the low protein expression of c-myc, bcl-2 and the high protein expression of p53, fas, fas-L and the cell factor TGF beta(1). The different inhibitory characteristics on the normal cells and cancer cells are possibly caused by gene and the cell factor expressions.
研究红毛五加多糖（AGP）对SGC-7901胃癌细胞的抑制作用及其可能机制。
采用细胞倍增时间分析、集落形成试验和MTT试验研究其抑制作用及特点。同时运用流式细胞术分析癌基因、抑癌基因及细胞因子的蛋白表达量。
AGP抑制SGC-7901细胞增殖及细胞集落形成能力。除最高剂量的AGP外（P<0.05），AGP对健康受试者外周血淋巴细胞及人胚腱细胞的活力和功能无抑制作用，最高剂量时对这两种正常细胞的活力和功能有轻微抑制。除AGP I和AGP III最低剂量外，AGP抑制SGC-7901细胞的活力和功能。AGP剂量与SGC-7901细胞之间存在剂量效应关系。AGP在分子水平的作用与c-myc和bcl-2基因蛋白低表达、p53、bax、fas和fas-L基因蛋白高表达以及细胞因子TGFβ(1)有关。AGP的抑制作用弱于顺铂，但强于维生素C。
红毛五加多糖通过c-myc、bcl-2蛋白低表达及p53、fas、fas-L蛋白高表达和细胞因子TGFβ(1)选择性抑制人胃癌细胞的增殖、集落形成能力以及活力和功能。对正常细胞和癌细胞不同的抑制特点可能由基因及细胞因子表达所致。
