通过环介导等温扩增检测商业猪用疫苗中的猪圆环病毒 1 型。
Detection of porcine circovirus type 1 in commercial porcine vaccines by loop-mediated isothermal amplification.
机构信息
Animal Health Research Institute, Council of Agriculture, Executive Yuan, No. 376, Chung-Cheng Road, Tansui, New Taipei City 25158, Taiwan.
出版信息
Folia Microbiol (Praha). 2011 Nov;56(6):483-9. doi: 10.1007/s12223-011-0072-7. Epub 2011 Sep 27.
A loop-mediated isothermal amplification (LAMP) method with a real-time monitoring system was developed for the detection of porcine circovirus type 1 (PCV1) in commercial swine vaccines. This method was highly specific for PCV1. No cross-reaction to porcine circovirus type 2, porcine parvovirus, pseudorabies virus, classical swine fever virus, and porcine reproductive and respiratory syndrome virus was observed. The analytical sensitivity of the LAMP for PCV1 DNA was 10 copies/μl in the case of positive recombinant plasmid comparable to that obtained from the nested polymerase chain reaction (nested PCR). Furthermore, 25 commercial swine vaccines were tested by both the LAMP and the nested PCR, and three of them were tested positive for PCV1 DNA. These results indicate that PCV1 DNA can be real-time detected by the LAMP; the method was highly specific, sensitive, and rapid for the detection of PCV1 DNA, particularly in commercial swine vaccines.
建立了一种实时监测环介导等温扩增（LAMP）方法，用于检测商用猪疫苗中的猪圆环病毒 1 型（PCV1）。该方法对 PCV1 具有高度特异性。与猪圆环病毒 2 型、猪细小病毒、伪狂犬病病毒、猪瘟病毒和猪繁殖与呼吸综合征病毒无交叉反应。LAMP 对阳性重组质粒的 PCV1 DNA 的分析灵敏度与巢式聚合酶链反应（nested PCR）相当，为 10 拷贝/μl。此外，用 LAMP 和嵌套 PCR 检测了 25 种商用猪疫苗，其中 3 种疫苗检测到 PCV1 DNA 阳性。这些结果表明，LAMP 可实时检测 PCV1 DNA；该方法对 PCV1 DNA 的检测具有高度特异性、敏感性和快速性，特别适用于商用猪疫苗。
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