Eser O, Eser B, Cosar M, Erdogan M O, Aslan A, Yıldız H, Solak M, Haktanır A
Department of Neurosurgery, Afyon Kocatepe University, Afyonkarahisar, Turkey.
Genet Mol Res. 2011 Aug 30;10(3):1923-30. doi: 10.4238/vol10-3gmr1222.
We investigated a possible association between aggrecan gene polymorphism and lumbar degenerative disc disease in Turkish patients. One hundred 20-30-year-old patients with or without low back pain were selected for the study. Lumbar magnetic resonance imaging was performed on all patients. The patient group had low back pain clinically and degenerative disc disease radiographically. The control group included patients with and without low back pain: all were negative radiographically for degenerative disc disease. Genomic DNA was extracted from all participants. A PCR assay were used to evaluate variable number of tandem repeat polymorphism of aggrecan gene alleles to determine if there was any correlation with degenerative disc disease. Significant associations were found between short repeated alleles of the aggrecan gene and severe disc degeneration. A significant association was also found between short repeated alleles of the aggrecan gene and multilevel disc herniation as well as extrusion and sequestration types of disc herniation. In Turkish population, short repeated alleles of the aggrecan gene are associated with increased disc degeneration and disc herniation.
我们研究了土耳其患者中聚集蛋白聚糖基因多态性与腰椎退行性椎间盘疾病之间可能存在的关联。选取了100名年龄在20至30岁之间、有或无腰痛的患者进行研究。对所有患者进行了腰椎磁共振成像检查。患者组临床上有腰痛，影像学上有椎间盘退变疾病。对照组包括有和无腰痛的患者：所有患者在影像学上均未发现椎间盘退变疾病。从所有参与者中提取基因组DNA。采用聚合酶链反应（PCR）检测法评估聚集蛋白聚糖基因等位基因的串联重复多态性数量变化，以确定其与椎间盘退变疾病是否存在相关性。研究发现，聚集蛋白聚糖基因的短重复等位基因与严重椎间盘退变之间存在显著关联。聚集蛋白聚糖基因的短重复等位基因与多节段椎间盘突出以及椎间盘突出的挤出型和游离型之间也存在显著关联。在土耳其人群中，聚集蛋白聚糖基因的短重复等位基因与椎间盘退变和椎间盘突出增加有关。
