Sram Radim J, Binkova Blanka, Beskid Olena, Milcova Alena, Rossner Pavel, Rossner Pavel, Rossnerova Andrea, Solansky Ivo, Topinka Jan
Institute of Experimental Medicine, Academy of Sciences of the Czech Republic (ASCR), 142 20 Prague, Czech Republic.
Air Qual Atmos Health. 2011 Dec;4(3-4):161-167. doi: 10.1007/s11869-011-0133-8. Epub 2011 Jan 29.
The effect of exposure to carcinogenic polycyclic aromatic hydrocarbons adsorbed onto respirable air particles (PM2.5, diameter < 2.5 μm) on DNA adducts and chromosomal aberrations was repeatedly studied in Prague, Czech Republic, in groups of policemen working in the downtown area and in bus drivers. Personal exposure was evaluated using personal samplers during working shifts. DNA adducts were analyzed in lymphocytes by the (32)P-postlabeling assay and chromosomal aberrations were analyzed by conventional cytogenetic analysis and fluorescent in situ hybridization (FISH). The impact of environmental pollution on DNA adducts and chromosomal aberrations was studied in a total of 950 subjects. Our results suggest that the environmental exposure of nonsmokers to concentrations higher than 1 ng benzo[a]pyrene/m(3) represents a risk of DNA damage, as indicated by an increase in DNA adducts and the genomic frequency of translocations determined by FISH.
在捷克共和国布拉格，针对在市中心工作的警察群体和公交司机群体，反复研究了暴露于吸附在可吸入空气颗粒物（PM2.5，直径<2.5微米）上的致癌多环芳烃对DNA加合物和染色体畸变的影响。在工作班次期间，使用个人采样器评估个人暴露情况。通过（32）P后标记分析法分析淋巴细胞中的DNA加合物，并通过传统细胞遗传学分析和荧光原位杂交（FISH）分析染色体畸变。总共对950名受试者研究了环境污染对DNA加合物和染色体畸变的影响。我们的结果表明，非吸烟者环境暴露于高于1纳克苯并[a]芘/立方米的浓度会带来DNA损伤风险，FISH测定的DNA加合物增加和易位基因组频率增加表明了这一点。
