程序性细胞死亡4蛋白的表达与喉鳞状细胞癌密切相关
[Expression of programmed cell death 4 protein is closely correlated with laryngeal squamous cell carcinomas].
作者信息
Wang Jianting, Zhang Yuxiang
机构信息
Department of Otorhinolaryngology Head and Neck Surgery, Beijing Chaoyang Hospital, Capital Medical University, Beijing, 100020, China.
出版信息
Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2011 Jun;25(12):539-41.
OBJECTIVE
To study whether programmed cell death 4 (PDCD4) is involved in the development and progression of laryngeal squamous cell carcinomas (LSCC).
METHOD
The expressions of PDCD4 protein were examined by immunohistochemical staining and Western blot in LSCC tissues and their surrounding laryngeal normal mucosa tissues.
RESULT
The positive rate of PDCD4 protein in LSCC was significantly lower than that in the laryngeal normal mucosa tissues by immunohistochemical staining (P<0.01). No correlation was found between positive expression of PDCD4 and gender, age, tumor site, TNM stage, but there was significant correlation between positive expression of PDCD4 from differentiation grading and lymphatic matastasis. Meanwhile,we got the similar results by Western blot (P<0.01).
CONCLUSION
The low expression of PDCD4 protein is closely related to the development and progression of LSCC. PDCD4 might be a novel molecular marker for prognosticating metastases and prognosis of LSCC.
目的
研究程序性细胞死亡4（PDCD4）是否参与喉鳞状细胞癌（LSCC）的发生发展过程。
方法
采用免疫组织化学染色和蛋白质印迹法检测LSCC组织及其周围喉正常黏膜组织中PDCD4蛋白的表达情况。
结果
免疫组织化学染色显示，LSCC中PDCD4蛋白的阳性率显著低于喉正常黏膜组织（P<0.01）。PDCD4的阳性表达与性别、年龄、肿瘤部位、TNM分期无关，但与分化程度和淋巴结转移有显著相关性。同时，蛋白质印迹法也得到了类似结果（P<0.01）。
结论
PDCD4蛋白低表达与LSCC的发生发展密切相关。PDCD4可能是预测LSCC转移和预后的新型分子标志物。
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