通过间接免疫荧光显微镜和免疫印迹法对一种抗波形蛋白的小鼠单克隆抗体进行表征。
Characterization of a mouse monoclonal antibody to vimentin by indirect immunofluorescence microscopy and immunoblotting.
作者信息
Fausser J L, Lesot H, Zidan G, Ruch J V
机构信息
Institut de Biologie Médicale, INSERM CJF 88-08, Université Louis Pasteur, Faculté de médecine, Strasbourg, France.
出版信息
J Biol Buccale. 1990 Mar;18(1):29-33.
PMID:2195017
Abstract
A murine IgM monoclonal antibody, termed MC15A13G was produced after immunization of Balb/c mouse with Swiss mouse dental papillae. This antibody, characterized by immunoblotting analysis and indirect immunofluorescence microscopy, recognized a 57 Kda protein identified as vimentin in different cell types with no cross reaction with other cytoskeletal proteins.
摘要
用瑞士小鼠牙乳头免疫Balb/c小鼠后，产生了一种名为MC15A13G的鼠IgM单克隆抗体。通过免疫印迹分析和间接免疫荧光显微镜鉴定，该抗体可识别一种57千道尔顿的蛋白质，在不同细胞类型中该蛋白质被确定为波形蛋白，且与其他细胞骨架蛋白无交叉反应。
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