Kasper M, Rudolf T, Verhofstad A A, Schuh D, Müller M
Institute of Pathology, Medical Academy Carl Gustav Carus, Dresden, Germany.
Histochemistry. 1993 Jul;100(1):65-71. doi: 10.1007/BF00268879.
The distribution of individual cytokeratin polypeptides in the adult rat lung parenchyma was investigated by immunohistochemistry with 44 monoclonal and 2 polyclonal antibodies. Simple epithelial cytokeratins 7, 8, 18 and 19 were found to be expressed differently in alveolar and bronchial epithelial cells. Three distinct types of alveolar cells were detected according to their pattern of immunoreactivity: type II cells strongly expressing cytokeratins 8 and 18 and weakly expressing cytokeratins 7 and 19 in the cell periphery; type I cells predominantly positive for cytokeratins 7 and 19 and weakly for cytokeratin 8; and a newly defined third cell type III (alveolar brush cell) with cytokeratin 18 abundantly expressed but organized in an unusual intracellular ("globular") structure. The latter cell type failed to bind the type II specific Maclura pomifera lectin, and contained no surfactant proteins. Bronchial epithelial cells exhibited a more or less uniform staining pattern for cytokeratins 8, 18 and 19 and focally for cytokeratins 4 and 7.
利用44种单克隆抗体和2种多克隆抗体，通过免疫组织化学方法研究了成年大鼠肺实质中各细胞角蛋白多肽的分布情况。发现简单上皮细胞角蛋白7、8、18和19在肺泡上皮细胞和支气管上皮细胞中的表达存在差异。根据免疫反应模式检测到三种不同类型的肺泡细胞：II型细胞在细胞周边强烈表达细胞角蛋白8和18，而细胞角蛋白7和19表达较弱；I型细胞主要对细胞角蛋白7和19呈阳性，对细胞角蛋白8呈弱阳性；还有一种新定义的III型细胞（肺泡刷细胞），其细胞角蛋白18大量表达，但以一种不寻常的细胞内（“球状”）结构存在。后一种细胞类型不能结合II型特异性桑科榕属凝集素，且不含表面活性蛋白。支气管上皮细胞对细胞角蛋白8、18和19呈现或多或少均匀的染色模式，对细胞角蛋白4和7呈局灶性染色。
