Henan Provincial Key Laboratory of Ion Beam Bio-engineering, Zhengzhou University, Henan, China.
Anim Sci J. 2011 Oct;82(5):642-53. doi: 10.1111/j.1740-0929.2011.00894.x. Epub 2011 Jun 16.
One hundred and twenty-six strains were isolated from corn stover in Henan Province, China, of which 105 isolates were considered to be lactic acid bacteria (LAB) according to Gram-positive, catalase-negative and mainly metabolic lactic acid product. Analysis of the 16S ribosomal DNA sequence of 21 representative strains was used to confirm the presence of the predominant groups and to determine the phylogenetic affiliation of isolates. The sequences from the various LAB isolates showed high degrees of similarity to those of the GenBank type strains between 99.4% and 100%. The prevalent LAB, predominantly Lactobacillus (85.6%), consisted of L. plantarum (33.3%), L. pentosus (28.6%) and L. brevis (23.7%). Other LAB species as Leuconostoc lactis (4.8%), Weissella cibaria (4.8%) and Enterococcus mundtii (4.8%) also presented in corn stover. The present study is the first to fully document corn stover-associated LAB involved in the silage fermentation. The identification results revealed LAB composition inhabiting corn stover and enabling the future design of appropriate inoculants aimed at improving the fermentation quality of silage.
从中国河南省的玉米秸秆中分离出 126 株菌株，其中 105 株根据革兰氏阳性、过氧化氢酶阴性和主要代谢乳酸产物被认为是乳酸菌（LAB）。对 21 株代表性菌株的 16S 核糖体 DNA 序列分析用于确认主要群体的存在，并确定分离株的系统发育归属。不同 LAB 分离株的序列与 GenBank 标准株之间的相似度高达 99.4%至 100%。主要的乳酸菌（85.6%）主要由植物乳杆菌（33.3%）、戊糖乳杆菌（28.6%）和短乳杆菌（23.7%）组成。其他乳酸菌物种，如乳球菌乳脂亚种（4.8%）、魏斯氏菌 cibaria（4.8%）和屎肠球菌（4.8%）也存在于玉米秸秆中。本研究首次全面记录了与青贮发酵相关的玉米秸秆相关乳酸菌。鉴定结果揭示了玉米秸秆中栖息的乳酸菌组成，为未来设计旨在提高青贮发酵质量的合适接种剂提供了依据。
