State Key Laboratory of Microbial Technology, Shandong University, Jinan, China.
Research and Development Department, Jinan Senkang Sanfeng Biological Engineering Co., Ltd, Jinan, China.
J Appl Microbiol. 2018 Sep;125(3):675-685. doi: 10.1111/jam.13914. Epub 2018 Jun 27.
Monitoring the succession of bacterial populations during corn stover ensiling is helpful for improving the silage quality.
Fermentation characteristics were assessed and bacterial communities were described along with the ensiling process. The ensiled corn stover exhibited chemical traits, such as low pH value (3·92 ± 0·02) and high levels of lactic acid (66·75 ± 1·97 g kg dry matter (DM)), which were associated with well-ensiled forages, as well as moderate concentrations of acetic acid (19·69 ± 1·51 g kg DM) and small amounts of 1,2-propanediol (4·4 ± 0·11 g kg DM). In the early stages of the ensiling process, a significant increase and then reduction in the abundance of species, Lactococcus lactis, Leuconostoc pseudomesenteroides, Pediococcus pentosaceus and Weissella sp., were observed. The species Lactobacillus plantarum group and Lactobacillus brevis grew vigorously, and the species Lactobacillus farciminis and Lactobacillus parafarraginis gradually increased along with the course of ensiling.
High-throughput sequencing was successfully used to describe bacterial communities throughout the process of corn stover ensiling.
The knowledge about the ecological succession of the dominant lactic acid bacteria could lead to improved ensiling practices and the selection of corn stover silage inoculants.
监测玉米秸秆青贮过程中细菌种群的演替有助于提高青贮饲料质量。
评估了发酵特性，并描述了细菌群落随青贮过程的变化。青贮玉米秸秆表现出化学特性，如低 pH 值（3.92±0.02）和高乳酸水平（66.75±1.97 g kg 干物质（DM）），这与青贮良好的饲料有关，同时还具有中等浓度的乙酸（19.69±1.51 g kg DM）和少量的 1,2-丙二醇（4.4±0.11 g kg DM）。在青贮过程的早期，观察到物种乳球菌（Lactococcus lactis）、肠膜明串珠菌（Leuconostoc pseudomesenteroides）、戊糖片球菌（Pediococcus pentosaceus）和魏斯氏菌（Weissella sp.）的丰度显著增加，然后减少。植物乳杆菌组（Lactobacillus plantarum group）和短乳杆菌（Lactobacillus brevis）生长旺盛，而类双歧杆菌（Lactobacillus farciminis）和副干酪乳杆菌（Lactobacillus parafarraginis）的物种则随着青贮过程的进行逐渐增加。
高通量测序成功地用于描述玉米秸秆青贮过程中细菌群落的变化。
了解优势乳酸菌的生态演替，可以改善青贮实践和选择玉米秸秆青贮接种剂。
