Department of Orthopedics & Physical Rehabilitation, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA.
Chem Asian J. 2011 Oct 4;6(10):2730-7. doi: 10.1002/asia.201100411. Epub 2011 Aug 24.
Strategies to encapsulate cells in cytocompatible three-dimensional hydrogels with tunable mechanical properties and degradability without harmful gelling conditions are highly desired for regenerative medicine applications. Here we reported a method for preparing poly(ethylene glycol)-co-polycarbonate hydrogels through copper-free, strain-promoted azide-alkyne cycloaddition (SPAAC) click chemistry. Hydrogels with varying mechanical properties were formed by "clicking" azido-functionalized poly(ethylene glycol)-co-polycarbonate macromers with dibenzocyclooctyne-functionalized poly(ethylene glycol) under physiological conditions within minutes. Bone marrow stromal cells encapsulated in these gels exhibited higher cellular viability than those encapsulated in photo-cross-linked poly(ethylene glycol) dimethacrylate. The precise control over the macromer compositions, cytocompatible SPAAC cross-linking, and the degradability of the polycarbonate segments make these hydrogels promising candidates for scaffold and stem cell assisted tissue repair and regeneration.
用于再生医学应用的策略是将细胞封装在具有可调机械性能和可降解性的细胞相容性三维水凝胶中，而不会产生有害的凝胶条件。在这里，我们报道了一种通过无铜应变促进叠氮化物-炔烃环加成（SPAAC）点击化学制备聚（乙二醇）-共-聚碳酸酯水凝胶的方法。通过在生理条件下将叠氮基功能化的聚（乙二醇）-共-聚碳酸酯大分子单体与二苯并环辛炔功能化的聚（乙二醇）点击，在数分钟内形成具有不同机械性能的水凝胶。与包封在光交联聚乙二醇二甲基丙烯酸酯中的细胞相比，包封在这些凝胶中的骨髓基质细胞表现出更高的细胞活力。对大分子单体组成、细胞相容性 SPAAC 交联和聚碳酸酯段的可降解性的精确控制使这些水凝胶成为支架和干细胞辅助组织修复和再生的有前途的候选物。
