Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095, USA.
Biomacromolecules. 2012 Oct 8;13(10):3013-7. doi: 10.1021/bm301346e. Epub 2012 Sep 12.
Oxime Click chemistry was used to form hydrogels that support cell adhesion. Eight-armed aminooxy poly(ethylene glycol) (PEG) was mixed with glutaraldehyde to form oxime-linked hydrogels. The mechanical properties, gelation kinetics, and water swelling ratios were studied and found to be tunable. It was also shown that gels containing the integrin ligand arginine-glycine-aspartic acid (RGD) supported mesenchymal stem cell (MSC) incorporation. High cell viability and proliferation of the encapsulated cells demonstrated biocompatibility of the material.
肟点击化学被用于形成支持细胞黏附的水凝胶。八臂氨氧基聚（乙二醇）（PEG）与戊二醛混合形成肟键合水凝胶。研究了其机械性能、凝胶动力学和水膨胀比，发现它们是可调的。还表明，含有整合素配体精氨酸-甘氨酸-天冬氨酸（RGD）的凝胶支持间充质干细胞（MSC）的掺入。包封细胞的高细胞活力和增殖证明了该材料的生物相容性。
