Department of Bioscience and Biotechnology, School of Physics, Konkuk University, Seoul 143-701, Korea.
J Am Chem Soc. 2011 Oct 26;133(42):16718-21. doi: 10.1021/ja2083587. Epub 2011 Sep 30.
We report on a new surface modification method for grafting a "dynamic" property for on-demand activation of the click reaction. Our approach utilizes the acetylene group masked with dicobalt hexacarbonyl, Co(2)(CO)(6), which is not reactive toward the click reaction. Electrochemical treatment reveals the acetylene group on the selected region, which is then used as a chemical handle for surface functionalization via the click reaction with an azide-containing molecule. Electrochemical and chemical conversions on the surface were verified by cyclic voltammetry, X-ray photoelectron spectroscopy, and fluorescence spectroscopy. We have demonstrated immobilization of an azide-modified RGD peptide and promotion of cell adhesion/migration to the region of electrochemical induction.
我们报告了一种新的表面修饰方法，用于赋予点击反应的按需激活“动态”特性。我们的方法利用二羰基合钴（Co(2)(CO)(6)）掩蔽的乙炔基团，该基团对点击反应没有反应性。电化学处理揭示了所选区域的乙炔基团，然后将其用作通过与含叠氮化物的分子的点击反应进行表面功能化的化学接头。通过循环伏安法、X 射线光电子能谱和荧光光谱验证了表面的电化学和化学转化。我们已经证明了通过电化学诱导区域固定化叠氮化物修饰的 RGD 肽和促进细胞黏附和迁移。
