Servicio de Resonancia Magnética Nuclear, Centro de Investigación Príncipe Felipe, Avenida Autopista del Saler 16, 46012 Valencia, Spain.
J Agric Food Chem. 2011 Nov 9;59(21):11407-16. doi: 10.1021/jf2030902. Epub 2011 Oct 7.
(13)C nuclear magnetic resonance (NMR) spectroscopy was explored as a simple and efficient technique for the quantitative analysis of nonpolar metabolites in plants. The method was first optimized with a mixture of known metabolites and then applied to the nonpolar leaf extracts of plants harvested in the Valencian community (eastern Spain) belonging to three different genera: Euphorbia (Euphorbiaceae), Araujia (Apocynaceae), and Morus (Moraceae). Furthermore, an exhaustive analysis of Euphorbia characias leaf and stem extracts from different geographic locations allowed that quantitative (13)C NMR spectroscopy is a suitable tool for metabolic profiling purpose.
（13）C 核磁共振（NMR）光谱被探索为一种简单有效的技术，用于对植物中非极性代谢物进行定量分析。该方法首先通过混合已知代谢物进行优化，然后应用于在西班牙巴伦西亚地区（西班牙东部）收获的三种不同属植物的非极性叶片提取物：Euphorbia（大戟科）、Araujia（夹竹桃科）和 Morus（桑科）。此外，对来自不同地理位置的 Euphorbia characias 叶片和茎提取物的详尽分析表明，定量（13）C NMR 光谱是代谢组学分析的合适工具。
