Institute for Micromanufacturing, Louisiana Tech University, Ruston, LA, USA.
Biosens Bioelectron. 2011 Dec 15;30(1):128-32. doi: 10.1016/j.bios.2011.08.042. Epub 2011 Sep 6.
A polymer nanostructured Fabry-Perot interferometer (FPI) based biosensor is reported. Different from a conventional FPI, the nanostructured FPI has a layer of Au-coated nanopores inside its cavity. The Au-coated nanostructure layer offers significant enhancement of optical transducing signals due to the localized surface plasmon resonance effect and also due to the significantly increased sensing surface area, which is up to at least two orders of magnitude larger than that of a conventional FPI-based biosensor. Using this technical platform, the immobilization of captures proteins (protein A) on the nanostructure layer and their binding with immunoglobulin G (IgG) has been monitored in real time, resulting in the shift of the interference fringes of the optical transducing signals. Current results show that the limit-of-detection of the biosensor should be lower than 10 pg/mL for IgG-protein A binding.
一种基于聚合物纳米结构法布里-珀罗干涉仪（FPI）的生物传感器被报道。与传统的 FPI 不同，纳米结构 FPI 在其腔体内有一层镀有 Au 的纳米孔。由于局域表面等离子体共振效应以及传感表面积的显著增加，Au 涂层纳米结构层提供了显著增强的光学转换信号，其传感表面积至少增加了两个数量级，比传统基于 FPI 的生物传感器大得多。利用这个技术平台，实时监测了纳米结构层上捕获蛋白（蛋白 A）的固定及其与免疫球蛋白 G（IgG）的结合，导致光学转换信号的干涉条纹发生位移。目前的结果表明，该生物传感器的检测限应该低于 10pg/mL 用于 IgG-蛋白 A 结合。
