Kang Kyoung Ah, Zhang Rui, Piao Mei Jing, Chae Sungwook, Kim Hee Sun, Park Jun Ho, Jung Kwang Sun, Hyun Jin Won
School of Medicine and Applied Radiological Science Research Institute, Jeju National University, Jeju, Korea.
Toxicol Ind Health. 2012 Jun;28(5):412-21. doi: 10.1177/0748233711413799. Epub 2011 Sep 28.
Baicalein (5,6,7-trihydroxyflavone) is a phenolic flavonoid compound derived mainly from the root of Scutellaria baicalensis Georgi, a medicinal plant traditionally used in oriental medicine. In our previous study, baicalein attenuated mitochondrial oxidative stress by scavenging reactive oxygen species (ROS) and by induction of nuclear factor erythroid 2-related factor 2 transcription factor-mediated manganese superoxide dismutase. In the present study, the protective effects of baicalein against oxidative stress-induced damage, especially cellular components including DNA, lipid, and protein, were studied. The results of this study showed that baicalein scavenged intracellular ROS. Baicalein inhibited the H₂O₂-induced DNA damage that was demonstrated by decreased phospho-H2A.X expression and DNA tail formation. In addition, it prevented the lipid peroxidation shown by the fluorescence intensity of diphenyl-1-pyrenylphosphine and the formation of thiobarbituric acid reactive substances. Moreover, baicalein inhibited protein oxidation demonstrated by protein carbonyl formation. Furthermore, baicalein protected cells via the inhibition of apoptosis induced by H₂O₂. The findings of this study suggest that baicalein provides protection for cellular components against oxidative damage via scavenging ROS and inhibiting apoptosis.
黄芩素（5,6,7-三羟基黄酮）是一种酚类黄酮化合物，主要来源于黄芩的根部，黄芩是一种传统上用于东方医学的药用植物。在我们之前的研究中，黄芩素通过清除活性氧（ROS）和诱导核因子红细胞2相关因子2转录因子介导的锰超氧化物歧化酶来减轻线粒体氧化应激。在本研究中，研究了黄芩素对氧化应激诱导的损伤，特别是对包括DNA、脂质和蛋白质在内的细胞成分的保护作用。本研究结果表明，黄芩素可清除细胞内ROS。黄芩素抑制了H₂O₂诱导的DNA损伤，这通过磷酸化H2A.X表达的降低和DNA尾部形成得以证明。此外，它还阻止了由二苯基-1-芘基膦荧光强度所示的脂质过氧化以及硫代巴比妥酸反应性物质的形成。此外，黄芩素抑制了由蛋白质羰基形成所证明的蛋白质氧化。此外，黄芩素通过抑制H₂O₂诱导的细胞凋亡来保护细胞。本研究结果表明，黄芩素通过清除ROS和抑制细胞凋亡为细胞成分提供抗氧化损伤保护。
