Bochniarz M, Wawron W
Department and Clinic of Animal Reproduction, Faculty of Veterinary Medicine, University of Life Sciences, Głeboka 30, 20-612 Lublin, Poland.
Pol J Vet Sci. 2011;14(3):405-10. doi: 10.2478/v10181-011-0060-5.
The aim of the present study was to evaluate the antibiotic susceptibility of methicillin-susceptible (MS) and methicillin-resistant (MR) coagulase-negative Staphylococcus (CNS) strains isolated from milk of cows with mastitis. The study was conducted on 100 CNS strains (20 MRCNS and 80 MSCNS) isolated from milk samples of 86 cows from the Lublin (Poland) region farms. Antibiotic susceptibility of microorganisms was evaluated using the disc-diffusion method on the Mueller-Hinton agar according to the guidelines of the National Committee for Clinical Laboratory Standards (NCCLS). The highest efficacy against MSCNS was demonstrated for cephalosporin antibiotics, i.e. cefacetril (91.3%), ceftiofur (67.5%), cefoperazone (66.3%) and cephalexin (60.0% of susceptible MSCNS strains). Moreover, a high percentage of vancomycin-susceptible strains was demonstrated (83.8%). The activity of combination of amoxicillin with clavulanic acid and gentamicin was found weaker (63.8% and 61.3% of susceptible strains, respectively). About 50.0% of MSCNS were susceptible to erythromycin, enrofloxacine and amoxicillin. A large proportion of CNS was resistant to neomycin, penicillin, tetracycline, streptomycin, lincomycin and ampicillin (28.8%, 30.0%, 31.3%, 31.3%, 33.8% and 33.8% of susceptible strains, respectively). The highest percentage of MRCNS was susceptible to vancomycin (75.0%), erythromycin (65.0%) and streptomycin (50.0%). Their susceptibility to enrofloxacine (35.0%) as well as gentamicin and tetracycline (30.0%) was markedly lower. The lowest activity was found for lincomycin and neomycin (20.0% of susceptible MRCNS strains, each).
本研究的目的是评估从患乳腺炎奶牛的乳汁中分离出的甲氧西林敏感（MS）和甲氧西林耐药（MR）凝固酶阴性葡萄球菌（CNS）菌株的抗生素敏感性。该研究对从波兰卢布林地区农场的86头奶牛的乳汁样本中分离出的100株CNS菌株（20株MR-CNS和80株MS-CNS）进行。根据美国国家临床实验室标准委员会（NCCLS）的指南，采用纸片扩散法在Mueller-Hinton琼脂上评估微生物的抗生素敏感性。头孢菌素类抗生素对MS-CNS的疗效最高，即头孢乙腈（91.3%）、头孢噻呋（67.5%）、头孢哌酮（66.3%）和头孢氨苄（60.0%的敏感MS-CNS菌株）。此外，还显示出高比例的万古霉素敏感菌株（83.8%）。发现阿莫西林与克拉维酸和庆大霉素联合使用的活性较弱（敏感菌株分别为63.8%和61.3%）。约50.0%的MS-CNS对红霉素、恩诺沙星和阿莫西林敏感。很大一部分CNS对新霉素、青霉素、四环素、链霉素、林可霉素和氨苄西林耐药（敏感菌株分别为28.8%、30.0%、31.3%、31.3%、33.8%和33.8%）。MR-CNS对万古霉素（75.0%）、红霉素（65.0%）和链霉素（50.0%）的敏感性最高。它们对恩诺沙星（35.0%）以及庆大霉素和四环素（30.0%）的敏感性明显较低。林可霉素和新霉素的活性最低（每种对敏感MR-CNS菌株的活性均为20.0%）。
