Park Young Kyung, Paik Young Hwan, Yoon Jang Won, Fox Lawrence K, Hwang Sun Young, Park Yong Ho
Department of Microbiology, College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University, Seoul 151-742, Korea.
J Vet Sci. 2013;14(3):299-305. doi: 10.4142/jvs.2013.14.3.299. Epub 2013 Jun 30.
The sequences of the ccrAB genes from bovine-, canine- and chicken-originating methicillin-resistant Staphylococcus (S.) epidermidis (MRSE) and bovine methicillin-resistant Staphylococcus (S.) aureus (MRSA) were compared to investigate the frequency of intra-species horizontal transfer of the staphylococcal cassette chromosome mec (SCCmec) complex. Nineteen MRSE strains were isolated from bovine milk, chickens, and dogs, and their genetic characteristics were investigated by multilocus sequence typing and SCCmec typing. Among the animal MRSE strains, the most frequent SCCmec type was type IV, which consisted of the type B mec complex and ccrAB type 2. The ccrA2 and ccrB2 genes were sequenced from the bovine, chicken and canine MRSE strains and compared with those of the bovine MRSA strains. The sequences generally clustered as MRSA and MRSE groups, regardless of the animal source. Additionally, no bovine MRSE sequence was associated with the bovine MRSA groups. Although most of the bovine MRSE and MRSA isolates possessed SCCmec type IV sequences, our results suggest that the intra-species gene transfer of the SCCmec complex between bovine S. aureus and bovine S. epidermidis strains is not a frequent event.
比较了源自牛、犬和鸡的耐甲氧西林表皮葡萄球菌（MRSE）及牛源耐甲氧西林金黄色葡萄球菌（MRSA）的ccrAB基因序列，以研究葡萄球菌盒式染色体mec（SCCmec）复合体种内水平转移的频率。从牛奶、鸡和狗中分离出19株MRSE菌株，并通过多位点序列分型和SCCmec分型研究其遗传特征。在动物MRSE菌株中，最常见的SCCmec类型是IV型，由B型mec复合体和ccrAB 2型组成。对牛、鸡和犬MRSE菌株的ccrA2和ccrB2基因进行测序，并与牛MRSA菌株的基因进行比较。无论动物来源如何，序列通常聚为MRSA和MRSE组。此外，没有牛MRSE序列与牛MRSA组相关。虽然大多数牛MRSE和MRSA分离株具有IV型SCCmec序列，但我们的结果表明，牛金黄色葡萄球菌和牛表皮葡萄球菌菌株之间SCCmec复合体的种内基因转移并非频繁发生。
