Bacteriology and Parasitology Division, National Veterinary Research and Quarantine Service, Anyang, Republic of Korea.
Foodborne Pathog Dis. 2011 Feb;8(2):231-8. doi: 10.1089/fpd.2010.0661. Epub 2010 Oct 30.
A total of 402 Staphylococcus aureus isolates from bovine mastitis milk collected during 2003-2009 in Korea were tested for susceptibility to 20 antimicrobial agents. All S. aureus isolates were susceptible to 11 of 20 antimicrobials tested; no resistance was observed against pirlimycin, telithromycin, novobiocin, penicillin/novobiocin, quinupristin/dalfopristin, clindamycin, rifampin, ciprofloxacin, trimethprim/sulfamethoxazol, vancomycin, and linezolid. Over 66% of the S. aureus isolates were resistant to penicillin. Resistance was also seen for gentamicin (11.9%), erythromycin (7.7%), methicillin (oxacillin and cefoxitin, 6.2%), and tetracycline (4.2%). No noticeable change was observed in penicillin, gentamicin, and erythromycin resistance over the 7-year period. Tetracycline resistance appeared to decrease consistently, whereas methicillin resistance was observed from 2005. About 2.7% (11/402) were resistant to three or more antimicrobials. Genotyping of the 17 methicillin-resistant S. aureus (MRSA) isolated from each cow revealed two staphylococcal cassette chromosome mec (SCCmec) types (IV and IVa), three spa types (t286, t324, and untypable), and two sequence types (ST1 and ST72). Eleven of 17 (64.7%) MRSA strains belonged to SCCmec IVa-t324-ST72. The rest of strains belonged to SCCmec IVa-t286-ST1 (n = 3) and SCCmec IV-untypable-ST72 (n = 3). None of the MRSA carried the Panton-Valentine leukocidin gene. These characteristics are the same as those found in community-acquired (CA) MRSA strains prevalent in humans in Korea. Three pulsed-field gel electrophoresis types (A-C) were observed among the 17 MRSA strains examined, and 14 strains belonged to the same pulsed-field gel electrophoresis pattern regardless of their geographical origin and year of isolation. The results of this study provide evidence of CA-MRSA infection in dairy cattle for the first time in Korea.
2003 年至 2009 年期间，从韩国奶牛乳腺炎奶样中分离的 402 株金黄色葡萄球菌对 20 种抗菌药物的敏感性进行了检测。所有金黄色葡萄球菌分离株对 20 种抗菌药物中的 11 种均敏感；未发现对吡利霉素、替考拉宁、新生霉素、青霉素/新生霉素、奎奴普丁/达福普汀、克林霉素、利福平、环丙沙星、复方新诺明/磺胺甲恶唑、万古霉素和利奈唑胺有耐药性。超过 66%的金黄色葡萄球菌分离株对青霉素耐药。对庆大霉素（11.9%）、红霉素（7.7%）、甲氧西林（苯唑西林和头孢西丁，6.2%）和四环素（4.2%）也有耐药性。7 年间，青霉素、庆大霉素和红霉素的耐药性没有明显变化。四环素耐药性似乎持续下降，而 2005 年出现了耐甲氧西林的情况。约 2.7%（11/402）对三种或三种以上抗菌药物耐药。对从每头牛分离的 17 株耐甲氧西林金黄色葡萄球菌（MRSA）进行基因分型，发现两种葡萄球菌盒染色体 mec（SCCmec）类型（IV 和 IVa）、三种 spa 类型（t286、t324 和无法分型）和两种序列类型（ST1 和 ST72）。17 株耐甲氧西林金黄色葡萄球菌中有 11 株（64.7%）属于 SCCmec IVa-t324-ST72。其余菌株属于 SCCmec IVa-t286-ST1（n=3）和 SCCmec IV-无法分型-ST72（n=3）。没有一株耐甲氧西林金黄色葡萄球菌携带杀白细胞素基因。这些特征与韩国流行的社区获得性（CA）耐甲氧西林金黄色葡萄球菌菌株相同。在检测的 17 株耐甲氧西林金黄色葡萄球菌中观察到三种脉冲场凝胶电泳类型（A-C），无论其地理来源和分离年份如何，14 株都属于相同的脉冲场凝胶电泳模式。本研究结果首次提供了韩国奶牛中社区获得性耐甲氧西林金黄色葡萄球菌感染的证据。
