Li Yanjiao, Yu Changning, Li Jiaolong, Zhang Lin, Gao Feng, Zhou Guanghong
College of Animal Science and Technology, Key Laboratory of Animal Origin Food Production and Safety Guarantee of Jiangsu Province, Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, Nanjing Agricultural University, Nanjing 210095, China.
College of Animal Science and Technology, Jiangxi Province Key Laboratory of Animal Nutrition, Jiangxi Agricultural University, Nanchang 330045, China.
Asian-Australas J Anim Sci. 2017 Dec;30(12):1764-1772. doi: 10.5713/ajas.17.0090. Epub 2017 Jun 26.
This study investigated the effects of different dietary energy sources on early postmortem muscle metabolism of finishing pigs.
Seventy-two barrow (Duroc×Landrace×Yorkshire, DLY) pigs (65.0±2.0 kg) were allotted to three iso-energetic and iso-nitrogenous diets: A (44.1% starch, 5.9% crude fat, and 12.6% neutral detergent fibre [NDF]), B (37.6% starch, 9.5% crude fat, and 15.4% NDF) or C (30.9% starch, 14.3% crude fat, and 17.8% NDF). After the duration of 28-day feeding experiment, 24 pigs (eight per treatment) were slaughtered and the M. longissimus lumborum (LL) samples at 45 min postmortem were collected.
Compared with diet A, diet C resulted in greater adenosine triphosphate and decreased phosphocreatine (PCr) concentrations, greater activity of creatine kinase and reduced percentage bound activities of hexokinase (HK), and pyruvate kinase (PK) in LL muscles (p<0.05). Moreover, diet C decreased the phosphor-AKT level and increased the hydroxy-hypoxia-inducible factor-1α (HIF-1α) level, as well as decreased the bound protein expressions of HK II, PKM2, and lactate dehydrogenase A (p<0.05).
Diet C with the lowest level of starch and the highest levels of fat and NDF could enhance the PCr utilization and attenuate glycolysis early postmortem in LL muscle of finishing pigs.
本研究探讨不同膳食能量来源对育肥猪宰后早期肌肉代谢的影响。
将72头阉公猪（杜洛克×长白×大白，DLY）（体重65.0±2.0千克）分配到三种等能量、等氮的日粮中：日粮A（44.1%淀粉、5.9%粗脂肪和12.6%中性洗涤纤维[NDF]）、日粮B（37.6%淀粉、9.5%粗脂肪和15.4%NDF）或日粮C（30.9%淀粉、14.3%粗脂肪和17.8%NDF）。经过28天的饲养试验后，宰杀24头猪（每种处理8头），并采集宰后45分钟的腰大肌（LL）样本。
与日粮A相比，日粮C导致三磷酸腺苷含量更高，磷酸肌酸（PCr）浓度降低，肌酸激酶活性更高，腰大肌中己糖激酶（HK）和丙酮酸激酶（PK）的结合活性百分比降低（p<0.05）。此外，日粮C降低了磷酸化AKT水平，增加了羟基化缺氧诱导因子-1α（HIF-1α）水平，并降低了HK II、PKM2和乳酸脱氢酶A的结合蛋白表达（p<0.05）。
淀粉水平最低、脂肪和NDF水平最高的日粮C可提高育肥猪宰后早期腰大肌中PCr的利用率并减弱糖酵解。
