Moorghen M, Ince P, Finney K J, Watson A J, Harris A L
Department of Pathology, University of Newcastle Upon Tyne, U.K.
Anticancer Res. 1990 May-Jun;10(3):605-11.
Nineteen samples of primary colorectal carcinoma and adjacent mucosa were examined for EGFr expression using radioligand binding assays and immunohistochemical staining with the monoclonal antibody EGFR1. Radioligand binding experiments showed expression of EGFr in both tumour and mucosa in all cases. In tumour samples EGFr levels ranged between 4 and 79 fmole per mg membrane protein (Kd = 0.1-0.4 X 10(-9) M). There was no significant difference in the level of EGFr expression between tumour and mucosa overall. Immunohistochemical staining with the EGFR1 antibody was useful in localising EGFr to epithelial elements although it was less sensitive than ligand binding assays.
使用放射性配体结合测定法和单克隆抗体EGFR1进行免疫组织化学染色，对19份原发性结直肠癌及其相邻黏膜样本进行EGFr表达检测。放射性配体结合实验表明，所有病例的肿瘤和黏膜中均有EGFr表达。在肿瘤样本中，EGFr水平为每毫克膜蛋白4至79飞摩尔（解离常数Kd = 0.1 - 0.4×10⁻⁹ M）。总体而言，肿瘤和黏膜之间的EGFr表达水平无显著差异。尽管EGFR1抗体免疫组织化学染色不如配体结合测定法敏感，但它有助于将EGFr定位到上皮成分。
