易于合成和多种 Nε-烷基赖氨酸肽的电离效率改变。
Facile synthesis and altered ionization efficiency of diverse Nε-alkyllysine-containing peptides.
机构信息
Tri-Institutional Training Program in Chemical Biology, Memorial Sloan-Kettering Cancer Center, New York, NY 10065, USA.
出版信息
Chem Commun (Camb). 2012 Feb 1;48(10):1514-6. doi: 10.1039/c1cc14711k. Epub 2011 Sep 30.
Abstract
Protein lysine methylation is a biologically-important posttranslational modification. Given the increased use of alkyllysine-containing peptides to study protein methylation, we describe an efficient solid-phase synthesis to access various alkyllysine-containing peptides. As an exemplary application, we evaluated how ionization efficiency of the alkyllysine peptides affects mass spectrometric analysis.
摘要
蛋白质赖氨酸甲基化是一种重要的生物翻译后修饰。鉴于越来越多地使用含烷基赖氨酸的肽来研究蛋白质甲基化，我们描述了一种有效的固相合成方法来获得各种含烷基赖氨酸的肽。作为一个示例应用，我们评估了烷基赖氨酸肽的离子化效率如何影响质谱分析。
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