Wang Yane-Shih, Wu Bo, Wang Zhiyong, Huang Ying, Wan Wei, Russell William K, Pai Pei-Jing, Moe Yin N, Russell David H, Liu Wenshe R
Department of Chemistry, Texas A&M University, College Station, TX 77843, USA.
Mol Biosyst. 2010 Sep;6(9):1557-60. doi: 10.1039/c002155e. Epub 2010 Mar 30.
A photocaged N(epsilon)-methyl-L-lysine has been genetically incorporated into proteins at amber codon positions in Escherichia coli using an evolved pyrrolysyl-tRNA synthetase-pylT pair. Its genetic incorporation and following photolysis to recover N(epsilon)-methyl-L-lysine at physiological pH provide a convenient method for the biosynthesis of proteins with monomethylated lysines at specific sites.
利用进化的吡咯赖氨酰 - tRNA合成酶 - pylT对，将光笼化的N（ε）-甲基 - L - 赖氨酸在大肠杆菌的琥珀密码子位置遗传掺入蛋白质中。其遗传掺入以及随后在生理pH下进行光解以回收N（ε）-甲基 - L - 赖氨酸，为在特定位点具有单甲基化赖氨酸的蛋白质的生物合成提供了一种便捷方法。
