Institute for Laser Medicine and Bio-photonics, School of Life Science & Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, 200240, China.
Lasers Med Sci. 2012 May;27(3):599-606. doi: 10.1007/s10103-011-0996-9. Epub 2011 Oct 1.
The aim of this study was to analyze the role of vascular endothelial growth factor (VEGF) in mechanisms of cutaneous remodeling induced by fractional CO(2) laser treatment. The dorsal skin of Kunming mice was exposed to a single-pass fractional CO(2) laser treatment. Biopsies were taken 1 h, and 1, 3, 7, 14, 28 and 56 days after treatment. Skin samples VEGF expression was evaluated by immunohistochemistry and ELISA, fibroblasts by hematoxylin-eosin staining, and types I and III collagen by ELISA. Staining for VEGF was found in many types of cell including fibroblasts. The amount of VEGF in the skin of laser-treated areas had increased significantly compared to that in the control areas on days 1 and 3 (P < 0.05, P < 0.01, respectively), then decreased by day 7 after treatment and returned to the baseline level. The number of fibroblasts in the skin of the laser-treated areas had increased significantly compared to that in control areas on days 3, 7, 14, 28 and 56 after irradiation (P < 0.05, P < 0.01, P < 0.01, P < 0.01, P < 0.01, respectively). The amount of type I collagen was significantly higher in the skin of the laser-treated areas compared to that in control areas from day 28 to day 56 (P < 0.05, respectively), and type III collagen was significantly higher from day 3 to day 56 (P < 0.05, P < 0.05, P < 0.05, P < 0.05, P < 0.01, respectively). There was a positive correlation between the level of VEGF and fibroblast proliferation early stage after laser treatment (r = 0.853, P < 0.01), but there was no correlation after the first week (r = -0.124, P > 0.05). The amounts of type I and III collagen showed no significant correlations with the expression of VEGF in the late stages after laser treatment (r = 0.417, P > 0.05 and r = 0.340, P > 0.05, respectively). The results suggest that VEGF might be mainly involved in the early stages of wound healing, including the stages of acute inflammation, fibroblast proliferation and vessel formation induced by fractional CO(2) laser resurfacing.
本研究旨在分析血管内皮生长因子（VEGF）在分 子 CO2 激光治疗诱导的皮肤重塑机制中的作用。昆明小鼠的背部皮肤接受单次分 子 CO2 激光治疗。在治疗后 1 小时、1 天、3 天、7 天、14 天、28 天和 56 天取皮肤标本。通过免疫组化和 ELISA 评估皮肤中 VEGF 的表达，通过苏木精-伊红染色评估成纤维细胞，通过 ELISA 评估 I 型和 III 型胶原。发现 VEGF 在包括成纤维细胞在内的多种细胞中染色。与对照组相比，激光治疗区皮肤中的 VEGF 量在第 1 天和第 3 天显著增加（P<0.05，P<0.01），然后在治疗后第 7 天下降，恢复到基线水平。与照射后第 3 天、7 天、14 天、28 天和 56 天的对照组相比，激光治疗区皮肤中的成纤维细胞数量显著增加（P<0.05，P<0.01，P<0.01，P<0.01，P<0.01）。从第 28 天到第 56 天，激光治疗区皮肤中的 I 型胶原量明显高于对照组（P<0.05），从第 3 天到第 56 天，III 型胶原量明显高于对照组（P<0.05，P<0.05，P<0.05，P<0.05，P<0.01）。在激光治疗后早期，VEGF 水平与成纤维细胞增殖呈正相关（r=0.853，P<0.01），但在第一周后无相关性（r=-0.124，P>0.05）。在激光治疗后晚期，I 型和 III 型胶原的量与 VEGF 的表达无显著相关性（r=0.417，P>0.05 和 r=0.340，P>0.05）。结果表明，VEGF 可能主要参与分 子 CO2 激光换肤诱导的创面愈合早期阶段，包括急性炎症、成纤维细胞增殖和血管形成阶段。
