Azab Walid, El-Sheikh Abuelyazeed, Abdel-Gawad Azza
Department of Virology, Zagazig University, Zagazig, Egypt.
Virus Genes. 2012 Feb;44(1):109-11. doi: 10.1007/s11262-011-0677-6. Epub 2011 Sep 29.
Equine herpesvirus 4 (EHV-4) is an important pathogen that causes respiratory tract disease in horse populations worldwide. Glycoprotein G (gG) homologs have been identified in several alphaherpesviruses as minor non-essential membrane-anchored glycoproteins. In this study, EHV-4 gG deletion mutant has been generated by using bacterial artificial chromosome technology to investigate the role of gG in viral pathogenesis. Our findings reported here revealed no significant difference between parental EHV-4 and gG-negative strain in their replication cycle in cell culture. Furthermore, virus titers and plaque formation were comparable in both viruses. It is noteworthy that these findings disagree with the previously published study describing gG deletion in another EHV-4 strain.
马疱疹病毒4型（EHV-4）是一种重要的病原体，可在全球马群中引起呼吸道疾病。在几种甲型疱疹病毒中已鉴定出糖蛋白G（gG）同源物，它们是次要的非必需膜锚定糖蛋白。在本研究中，通过使用细菌人工染色体技术构建了EHV-4 gG缺失突变体，以研究gG在病毒致病机制中的作用。我们在此报告的研究结果显示，亲本EHV-4和gG阴性毒株在细胞培养中的复制周期没有显著差异。此外，两种病毒的病毒滴度和噬斑形成情况相当。值得注意的是，这些发现与先前发表的关于另一株EHV-4中gG缺失的研究结果不一致。
