Downey K M, Tan C K, So A G
Department of Medicine, University of Miami School of Medicine, FL 33101.
Bioessays. 1990 May;12(5):231-6. doi: 10.1002/bies.950120507.
During the past few years significant progress has been made in our understanding of the structure and function of the proteins involved in eukaryotic DNA replication. Data from several laboratories suggest that, in contrast to prokaryotic DNA replication, two distinct DNA polymerases are required for eukaryotic DNA replication, i.e. DNA polymerase delta for the synthesis of the leading strand and DNA polymerase alpha for the lagging strand. Several accessory proteins analogous to prokaryotic replication factors have been identified and some of these are specific for pol delta whereas others affect both DNA replicases. The replicases and their accessory proteins appear to be highly conserved in eukaryotes, as homologous proteins have been found in species ranging from humans to yeast.
在过去几年中，我们对真核生物DNA复制所涉及蛋白质的结构和功能的理解取得了重大进展。来自多个实验室的数据表明，与原核生物DNA复制不同，真核生物DNA复制需要两种不同的DNA聚合酶，即用于前导链合成的DNA聚合酶δ和用于后随链合成的DNA聚合酶α。已经鉴定出几种类似于原核生物复制因子的辅助蛋白，其中一些对聚合酶δ具有特异性，而其他一些则影响两种DNA复制酶。这些复制酶及其辅助蛋白在真核生物中似乎高度保守，因为在从人类到酵母的各种物种中都发现了同源蛋白。
