School of Medical Sciences, RMIT University, P.O. Box 71, Bundoora, VIC 3083, Australia.
Oxid Med Cell Longev. 2011;2011:728645. doi: 10.1155/2011/728645. Epub 2011 Sep 22.
UV-induced inflammation and reactive oxygen species formation are involved in the development of melanoma. Natural products like 5β-scymnol and CO(2)-supercritical fluid extract (CO(2)-SFE) of mussel oil contain anti-inflammatory and antioxidant properties that may aid in reducing the deleterious effects of UV radiation. Therefore, their effect on the release of the proinflammatory cytokine, tumour necrosis factor-α (TNF-α), from UVB-irradiated human melanocytic cells was examined. Human epidermal melanocytes (HEM) and MM96L melanoma cells were exposed to UVB radiation and IL-1α. Cell viability and TNF-α levels were determined 24 hours after-irradiation while p38 mitogen-activated protein kinase (MAPK) activation was observed at 15 min after-irradiation. When α-tocopherol, CO(2)-SFE mussel oil, and 5β-scymnol were added to the UVB-irradiated HEM cells treated with IL-1α, TNF-α levels fell by 53%, 65%, and 76%, respectively, while no inhibition was evident in MM96L cells. This effect was not due to inhibition of the intracellular p38 MAPK signalling pathway. These compounds may be useful in preventing inflammation-induced damage to normal melanocytes.
UV 诱导的炎症和活性氧形成参与了黑色素瘤的发展。像 5β-石竹烯和贻贝油的 CO2 超临界流体提取物（CO2-SFE）这样的天然产物具有抗炎和抗氧化特性，可能有助于减少 UV 辐射的有害影响。因此，研究了它们对 UVB 照射的人黑素细胞中促炎细胞因子肿瘤坏死因子-α（TNF-α）释放的影响。将人表皮黑素细胞（HEM）和 MM96L 黑色素瘤细胞暴露于 UVB 辐射和 IL-1α 下。在照射后 24 小时测定细胞活力和 TNF-α 水平，而在照射后 15 分钟观察到 p38 丝裂原活化蛋白激酶（MAPK）的激活。当 α-生育酚、CO2-SFE 贻贝油和 5β-石竹烯被添加到用 IL-1α 处理的 UVB 照射的 HEM 细胞中时，TNF-α 水平分别下降了 53%、65%和 76%，而 MM96L 细胞中没有明显的抑制作用。这种作用不是由于抑制细胞内 p38 MAPK 信号通路。这些化合物可能有助于预防炎症引起的正常黑素细胞损伤。
