三氮唑修饰的 DNA 模板体外转录的高效 RNA 合成。

Efficient RNA synthesis by in vitro transcription of a triazole-modified DNA template.

机构信息

School of Chemistry, University of Southampton, Highfield, Southampton, SO17 1BJ, UK.

出版信息

Chem Commun (Camb). 2011 Nov 28;47(44):12057-8. doi: 10.1039/c1cc14316f. Epub 2011 Sep 30.

PMID:21961113

Abstract

A DNA strand containing a triazole phosphodiester mimic is an efficient template for in vitro transcription. This is the first demonstration of transcription through a heavily modified DNA backbone linkage and it suggests that click-ligated DNA could be useful for the direct synthesis of biologically active RNA and proteins.

摘要

含三唑磷酸二酯模拟物的 DNA 链是体外转录的有效模板。这是首次通过高度修饰的 DNA 主链连接实现转录的证明，这表明点击连接的 DNA 可用于生物活性 RNA 和蛋白质的直接合成。