El-Sagheer Afaf H, Brown Tom
Department of Chemistry,University of Oxford,Chemistry Research Laboratory,12 Mansfield Road,Oxford,OX1 3TA,UK.
Q Rev Biophys. 2015 Nov;48(4):429-36. doi: 10.1017/S0033583515000141.
We describe the development of a chemical process based on the CuAAC reaction (click chemistry) to ligate DNA strands and produce an unnatural triazole backbone linkage. The chemical reaction is templated by a complementary DNA splint which accelerates the reaction and provides the required specificity. The resultant 1,4-triazole linkage is read through by DNA and RNA polymerases and is biocompatible in bacterial and human cells. This work has implications for the synthesis of chemically modified genes and other large modified DNA and RNA constructs.
我们描述了一种基于铜催化的叠氮化物-炔烃环加成反应（点击化学）的化学过程的开发，用于连接DNA链并产生一种非天然的三唑主链连接。该化学反应由互补的DNA夹板作为模板，它加速了反应并提供了所需的特异性。所得的1,4-三唑连接可被DNA和RNA聚合酶识别，并且在细菌和人类细胞中具有生物相容性。这项工作对化学修饰基因以及其他大型修饰DNA和RNA构建体的合成具有重要意义。
