Department of Basic Sciences and Environment, University of Copenhagen, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Denmark.
J Agric Food Chem. 2011 Nov 9;59(21):11735-43. doi: 10.1021/jf2027632. Epub 2011 Oct 12.
The area cultivated with Artemisia annua for the extraction of the antimalarial compound artemisinin is increasing, but the environmental impact of this cultivation has not yet been studied. A sensitive and robust method using liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed for the determination of artemisinin in soil. Dihydroartemisinin and artemether were included in the method, and performance on analytical columns of both traditional C(18) phenyl-hexyl and porous shell particles-based Kinetex types was characterized. The versatility of the method was demonstrated on surface water and groundwater samples and plant extracts. The limit of detection was 55, 30 (25 ng/g soil), and 4 ng/mL for dihydroartemisinin, artemisinin, and artemether, respectively. Method performance was demonstrated using naturally contaminated soil samples from A. annua fields in Kenya. The highest observed concentrations were above EC(10) for lettuce growth. Monitoring of artemisinin in soil with A. annua crop production seems necessary to further understand the impact in the environment.
青蒿素提取用黄花蒿种植面积在不断增加，但这种种植方式对环境的影响尚未得到研究。本研究建立了一种利用液相色谱-串联质谱（LC-MS/MS）测定土壤中青蒿素的灵敏、稳健的方法。该方法同时测定了双氢青蒿素和青蒿琥酯，对传统 C(18)苯基-己基和多孔壳颗粒型 Kinetex 两种分析柱的性能进行了表征。该方法通用性强，可用于地表水和地下水样品及植物提取物的分析。双氢青蒿素、青蒿素和青蒿琥酯的检测限分别为 55、30（25 ng/g 土壤）和 4 ng/mL。该方法使用肯尼亚青蒿种植地受污染土壤样品进行了验证。观察到的最高浓度均高于生菜生长的 EC(10)。随着青蒿素作物的种植，对土壤中青蒿素进行监测对进一步了解其对环境的影响非常必要。
