Section of Organic Chemistry and Biochemistry, Department of Chemistry, University of Ioannina, Ioannina, Greece.
Magn Reson Chem. 2020 Mar;58(3):232-244. doi: 10.1002/mrc.4971. Epub 2020 Jan 7.
Artemisia annua is a promising and potent antimalarial herbal drug. This activity has been ascribed to its component artemisinin, a sesquiterpene lactone. The ability to determine artemisinin and its known analogs in plant extracts is an especially difficult task because the compounds are present in low concentrations, are thermolabile, and lack ultraviolet or fluorescent chromophores. We report herein a facile and rapid 1-D H, 1-D total correlation spectroscopy, 2-D H- C heteronuclear single quantum coherence, and H- C heteronuclear multiple bond correlation nuclear magnetic resonance techniques for the simultaneous identification and quantification of artemisinin and five of its analogs along with five flavonoids, an aromatic ketone, and camphor (in total, 13 compounds) in crude diethyl ether A. annua extract without the need of laborious isolation of the individual analytes. The above method was validated in terms of precision, linearity, and limit of detection. The analytical results were found to be in excellent agreement with those obtained with the use of the time consuming high-performance liquid chromatography with diode-array detection and liquid chromatography with tandem mass spectrometry for the compounds that standards were available.
青蒿是一种很有前途的抗疟草药。这种活性被归因于其成分青蒿素，一种倍半萜内酯。因为这些化合物的浓度低，热不稳定，缺乏紫外或荧光发色团，所以能够在植物提取物中确定青蒿素及其已知类似物是一项特别困难的任务。我们在此报告了一种简便快捷的一维氢- 1 维总相关光谱、二维氢-碳异核单量子相干和氢-碳异核多键相关核磁共振技术，可用于同时鉴定和定量分析青蒿素及其五种类似物以及五种类黄酮、一种芳香酮和樟脑（总共 13 种化合物），无需费力分离各个分析物。该方法在精密度、线性和检测限方面得到了验证。对于有标准品的化合物，分析结果与使用费时的二极管阵列检测高效液相色谱法和串联质谱液相色谱法得到的结果非常吻合。
