School of Traditional Chinese Medicine, Capital Medical University, No.10, Xitoutiao, You'anmenwai, Fengtai District, Beijing 100069, China.
Molecules. 2019 Apr 18;24(8):1530. doi: 10.3390/molecules24081530.
: To develop an accurate and rapid ultra-performance liquid chromatography (UPLC) coupled with a photodiode array (PDA) method for the simultaneous determination of artemisinin (Art), arteannuin B (Art B), arteannuin C (Art C), dihydroartemisinic acid (DHAA) and artemisinic acid (AA) in L. : Chromatography separation was performed on an ACQUITY UPLC BEH C18 Column with isocratic elution; the mobile phase was 0.1% formic acid aqueous solution (A) and acetonitrile (B) (A:B = 40:60, /). Data were recorded at an ultraviolet (UV) wavelength of 191 nm for Art, Art C, DHAA and AA, and 206 nm for Art B. : The calibration curves of the five sesquiterpene components were all linear with correlation coefficients more than 0.9990. The linear ranges were 31.44-1572 μg/mL, 25.48-1274 μg/mL, 40.56-2028 μg/mL, 31.44-1572 μg/mL and 26.88-1396 μg/mL for Art, Art B, Art C, DHAA and AA, respectively. The precision ranged from 0.08% to 2.88%, the stability was from 0.96% to 1.66%, and the repeatability was all within 2.42% and had a mean extraction recovery of 96.5% to 100.6%. : The established UPLC-PDA method would be valuable for improving the quantitative analysis of sesquiterpene components in L.
建立一个准确且快速的超高效液相色谱（UPLC）与光电二极管阵列（PDA）方法，用于同时测定青蒿（Art）、青蒿素 B（Art B）、青蒿素 C（Art C）、二氢青蒿酸（DHAA）和青蒿酸（AA）在青蒿中的含量。
色谱分离在 ACQUITY UPLC BEH C18 柱上进行，采用等度洗脱；流动相为 0.1%甲酸水溶液（A）和乙腈（B）（A:B=40:60，/）。Art、Art C、DHAA 和 AA 的紫外（UV）波长为 191nm，Art B 的 UV 波长为 206nm 记录数据。
五种倍半萜类成分的校准曲线均呈线性，相关系数均大于 0.9990。线性范围分别为 Art 31.44-1572μg/mL、Art B 25.48-1274μg/mL、Art C 40.56-2028μg/mL、DHAA 31.44-1572μg/mL 和 AA 26.88-1396μg/mL。精密度范围为 0.08%-2.88%，稳定性范围为 0.96%-1.66%，重复性均在 2.42%以内，平均提取回收率为 96.5%-100.6%。
所建立的 UPLC-PDA 方法将有助于提高青蒿中倍半萜类成分的定量分析。
