Hacettepe University, Chemical Engineering Department, Ankara, Turkey; Cumhuriyet University, Chemical Engineering Department, Sivas, Turkey.
J Colloid Interface Sci. 2012 Jan 1;365(1):63-71. doi: 10.1016/j.jcis.2011.08.071. Epub 2011 Sep 10.
In this study, click chemistry was proposed as a tool for tuning the surface hydrophilicity of monodisperse-macroporous particles in micron-size range. The monodisperse-porous particles carrying hydrophobic or hydrophilic molecular brushes on their surfaces were obtained by the proposed modification. Hydrophilic poly(glycidyl methacrylate-co-ethylene dimethacrylate), poly(GMA-co-EDM) particles were hydrophobized by the covalent attachment of poly(octadecyl acrylate-co-propargyl acrylate), poly(ODA-co-PA) copolymer onto the particle surface via triazole formation by click chemistry. In the second part, Hydrophobic poly(4-chloromethylstyrene-co-divinylbenzene), poly(CMS-co-DVB) particles were hydrophilized by the covalent attachment of poly(vinyl alcohol), PVA onto their surface also via triazole formation by click chemistry. The presence of PVA and poly(ODA-co-PA) copolymer on the corresponding particles was shown by FTIR-DRS. After click-coupling reactions applied for both hydrophobic poly(CMS-co-DVB) and hydrophilic poly(GMA-co-EDM) particles, the marked changes in surface polarity were shown by contact angle measurements. Protein adsorption characteristics of plain and modified particles were investigated for both materials. In the isoelectric point of albumin, the non-specific albumin adsorption decreased from 225 to 80 mg/g by grafting PVA onto the poly(CMS-co-DVB) beads. On the other hand, the non-specific albumin adsorption onto the plain poly(GMA-co-EDM) beads increased from 50 to 400 mg/g by the covalent attachment of poly(ODA-co-PA) copolymer onto the bead-surface via click chemistry. The protein adsorption behavior was efficiently regulated by the covalent attachment of appropriate molecular brushes onto the surfaces of selected particles. The results indicated that "click chemistry" was an efficient tool for controlling the polarity of monodisperse-macroporous particles.
在这项研究中，点击化学被提议作为一种工具，用于调整单分散大孔颗粒在微米范围内的表面亲水性。通过所提出的修饰，获得了表面带有疏水性或亲水性分子刷的单分散多孔颗粒。亲水性聚（甲基丙烯酸缩水甘油酯-共-乙二醇二甲基丙烯酸酯），聚（GMA-co-EDM）颗粒通过点击化学通过三唑形成将聚（十八烯丙烯酸酯-共-丙烯酰基丙炔酸酯），聚（ODA-co-PA）共聚物共价键合到颗粒表面而疏水化。在第二部分中，通过点击化学通过三唑形成，将疏水性聚（4-氯甲基苯乙烯-共-二乙烯基苯），聚（CMS-co-DVB）颗粒的共价键合到其表面上的聚（聚乙烯醇），PVA 亲水化。通过傅里叶变换红外光谱 - 漫反射（FTIR-DRS）证明了相应颗粒上存在 PVA 和聚（ODA-co-PA）共聚物。在应用于疏水性聚（CMS-co-DVB）和亲水性聚（GMA-co-EDM）颗粒的点击偶联反应之后，通过接触角测量显示了表面极性的明显变化。研究了两种材料的普通和改性颗粒的蛋白质吸附特性。在白蛋白的等电点处，通过将 PVA 接枝到聚（CMS-co-DVB）珠上，非特异性白蛋白吸附从 225 至 80mg/g 降低。另一方面，通过点击化学将聚（ODA-co-PA）共聚物共价键合到珠表面上，将普通的聚（GMA-co-EDM）珠上的非特异性白蛋白吸附从 50 至 400mg/g 增加。通过将合适的分子刷共价键合到选定颗粒的表面上，可以有效地调节蛋白质吸附行为。结果表明，“点击化学”是一种有效控制单分散大孔颗粒极性的工具。
