量子点增强化学发光检测用于毛细管电泳同时测定多巴胺和肾上腺素。
Quantum dot-enhanced chemiluminescence detection for simultaneous determination of dopamine and epinephrine by capillary electrophoresis.
机构信息
Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education), College of Chemistry and Chemical Engineering, Guangxi Normal University, Guilin, 541004, China.
出版信息
Talanta. 2011 Oct 15;85(5):2650-4. doi: 10.1016/j.talanta.2011.08.032. Epub 2011 Aug 22.
A sensitive method based on quantum dot (QD)-enhanced capillary electrophoresis-chemiluminescence (CE-CL) detection was developed for simultaneous determination of dopamine (DA) and epinephrine (E). In this work, CdTe QD was added into the running buffer of CE to catalyze the post-column CL reaction between luminol and hydrogen peroxide, achieving higher CL emission. Negative peaks were produced due to the inhibitory effects on CL emission from DA and E eluted from the electrophoretic capillary. The decrease in CL intensity was proportional to the concentration of DA and E in the range of 8.0 × 10(-8)-5.0 × 10(-6)M and 4.0 × 10(-8)-5.0 × 10(-6)M, respectively. Detection limits for DA and E were 2.3 × 10(-8)M and 9.3 × 10(-9)M, respectively. Using this method, the levels of DA and E in human urine from healthy donors were determined.
一种基于量子点（QD）增强毛细管电泳-化学发光（CE-CL）检测的灵敏方法被开发出来，用于同时测定多巴胺（DA）和肾上腺素（E）。在这项工作中，将 CdTe QD 添加到 CE 的运行缓冲液中，以催化鲁米诺和过氧化氢之间的柱后 CL 反应，从而实现更高的 CL 发射。由于 DA 和 E 从电泳毛细管中洗脱出来对 CL 发射的抑制作用，产生了负峰。CL 强度的降低与 DA 和 E 的浓度成正比，范围分别为 8.0×10(-8)-5.0×10(-6)M 和 4.0×10(-8)-5.0×10(-6)M。DA 和 E 的检测限分别为 2.3×10(-8)M 和 9.3×10(-9)M。使用该方法，测定了来自健康供体的人尿中 DA 和 E 的水平。
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