Merli Daniele, Cutaia Alessandra, Hallulli Ines, Bonanni Alessandra, Alberti Giancarla
Department of Chemistry, University of Pavia, Via Taramelli 12, 27100 Pavia, Italy.
Polymers (Basel). 2024 Sep 6;16(17):2528. doi: 10.3390/polym16172528.
This paper introduces a quantitative method for dopamine determination. The method is based on a molecularly imprinted polypyrrole (e-MIP)-modified screen-printed electrode, with differential pulse voltammetry (DPV) as the chosen measurement technique. The dopamine molecules are efficiently entrapped in the polymeric film, creating recognition cavities. A comparison with bare and non-imprinted polypyrrole-modified electrodes clearly demonstrates the superior sensitivity, selectivity, and reproducibility of the e-MIP-based one; indeed, a sensitivity of 0.078 µA µM, a detection limit (LOD) of 0.8 µM, a linear range between 0.8 and 45 µM and a dynamic range of up to 350 µM are achieved. The method was successfully tested on fortified synthetic and human urine samples to underline its applicability as a screening method for biomedical tests.
本文介绍了一种测定多巴胺的定量方法。该方法基于分子印迹聚吡咯（e-MIP）修饰的丝网印刷电极，并选用差分脉冲伏安法（DPV）作为测量技术。多巴胺分子有效地包埋在聚合物膜中，形成识别空腔。与裸电极和非印迹聚吡咯修饰电极的比较清楚地表明了基于e-MIP电极具有更高的灵敏度、选择性和重现性；实际上，实现了0.078 μA μM的灵敏度、0.8 μM的检测限（LOD）、0.8至45 μM的线性范围以及高达350 μM的动态范围。该方法在加标的合成尿液和人体尿液样本上成功进行了测试，以强调其作为生物医学检测筛选方法的适用性。
