Department of Biochemistry, Centre for Biological Science, K.S.R. College of Arts and Science, Thokkavadi, Tiruchengode, Tamil Nadu, 637215, India.
Int J Cardiol. 2013 May 25;165(3):494-8. doi: 10.1016/j.ijcard.2011.09.005. Epub 2011 Sep 29.
Cardiovascular diseases (CVDs) have a high prevalence in developing and developed countries and myocardial infarction accounts for majority of deaths and disabilities. The current study dealt with the protective role of Amaranthus viridis Linn on isoproterenol (ISO)-induced myocardial infarction (MI) in rats.
Subcutaneous injection of ISO (20 mg/kg body weight in 1 ml saline) to rats for two consecutive days offered significant alteration in cardiac marker enzymes (AST, ALT, LDH and CPK), cardiac troponin, lipid peroxidation products (TBARS and hydroperoxide) and antioxidant system (CAT, SOD, GPx, GST, GSH and GSSG). ISO-induced myocardial damage was indicated by increased activities of marker enzymes in serum and the levels of cardiac troponin in the serum. In addition to these diagnostic markers, the levels of lipid peroxidation products in the heart were significantly (p<0.05) increased and the activities of enzymic antioxidants and non-enzymic antioxidant such as glutathione in the heart was significantly (p<0.05) decreased and GSSG in the heart was increased in ISO-induced rats.
Effect of Amaranthus viridis oral treatment (100, 200 and 300 mg/kg body weight) for 45 days elicited a significant cardio protective activity by lowering the levels of serum marker enzymes, cardiac troponin, GSSG and lipid peroxidation and elevated the levels of antioxidant enzymes and GSH. The effect at a dose of 300 mg/kg of A. viridis was more pronounced than that of the dose 100 mg/kg and 200mg/kg and brought back all the parameters to near normal. The effect produced by A. viridis was compared with α-tocopherol.
The present findings have demonstrated that the cardioprotective effects of A. viridis in ISO-induced oxidative damage may be due to an augmentation of the endogenous antioxidants and inhibition of lipid peroxidation of membrane.
心血管疾病（CVDs）在发展中国家和发达国家都有很高的发病率，心肌梗死占大多数死亡和残疾。本研究探讨了苋菜（Amaranthus viridis Linn）对大鼠异丙肾上腺素（ISO）诱导的心肌梗死（MI）的保护作用。
连续两天向大鼠皮下注射 ISO（20mg/kg 体重，1ml 盐水中），导致心脏标志物酶（AST、ALT、LDH 和 CPK）、心肌肌钙蛋白、脂质过氧化产物（TBARS 和氢过氧化物）和抗氧化系统（CAT、SOD、GPx、GST、GSH 和 GSSG）发生显著改变。ISO 诱导的心肌损伤表现为血清标志物酶活性升高和血清中心肌肌钙蛋白水平升高。除了这些诊断标志物外，心脏中的脂质过氧化产物水平显著升高（p<0.05），心脏中的酶抗氧化剂和非酶抗氧化剂如谷胱甘肽的活性显著降低（p<0.05），ISO 诱导的大鼠心脏中的 GSSG 增加。
苋菜口服治疗（100、200 和 300mg/kg 体重）45 天的效果通过降低血清标志物酶、心肌肌钙蛋白、GSSG 和脂质过氧化水平，提高抗氧化酶和 GSH 水平，显著发挥了心脏保护作用。剂量为 300mg/kg 的 A. viridis 的效果比 100mg/kg 和 200mg/kg 的效果更明显，使所有参数恢复接近正常。A. viridis 的作用与 α-生育酚进行了比较。
本研究结果表明，A. viridis 在 ISO 诱导的氧化损伤中的心脏保护作用可能是由于内源性抗氧化剂的增加和膜脂质过氧化的抑制。
