Department of Biochemistry and Biotechnology, Annamalai University, Annamalai Nagar 608 002, Tamil Nadu, India.
J Biochem Mol Toxicol. 2012 Sep;26(9):368-73. doi: 10.1002/jbt.21431. Epub 2012 Aug 13.
This study evaluates the protective effects of thymol on altered plasma lipid peroxidation products and nonenzymic antioxidants in isoproterenol (ISO)-induced myocardial infarcted rats. Male albino Wistar rats were pre and cotreated with thymol (7.5 mg/kg body weight) daily for 7 days. ISO (100 mg/kg body weight) was subcutaneously injected into rats on 6th and 7th day to induce myocardial infarction (MI). Increased activity/levels of serum creatine kinase-MB (CK-MB), plasma thiobarbituric acid reactive substances, lipid hydroperoxides, and conjugated dienes with decreased levels of plasma reduced glutathione (GSH), vitamin C, and vitamin E were observed in ISO-induced myocardial infarcted rats. Pre and cotreatment with thymol (7.5 mg/kg body weight) showed normalized activity of serum CK-MB and near normalized levels of plasma lipid peroxidation products, reduced GSH, vitamin C, and vitamin E in myocardial infarcted rats. Furthermore, the in vitro study on reducing power of thymol confirmed its potent antioxidant action. Thus, thymol protects ISO-induced MI in rats by its antilipid peroxidation and antioxidant properties.
本研究评价了百里香酚对异丙肾上腺素（ISO）诱导的心肌梗死大鼠血浆脂质过氧化产物和非酶抗氧化剂的保护作用。雄性白化 Wistar 大鼠预先和同时每日用百里香酚（7.5mg/kg 体重）处理 7 天。在第 6 天和第 7 天，ISO（100mg/kg 体重）被皮下注射到大鼠体内以诱导心肌梗死（MI）。在 ISO 诱导的心肌梗死大鼠中，观察到血清肌酸激酶同工酶-MB（CK-MB）、血浆硫代巴比妥酸反应性物质、脂质过氧化物、共轭二烯的活性/水平升高，而血浆还原型谷胱甘肽（GSH）、维生素 C 和维生素 E 的水平降低。预先和同时用百里香酚（7.5mg/kg 体重）处理可使心肌梗死大鼠的血清 CK-MB 活性正常化，并使血浆脂质过氧化产物、GSH、维生素 C 和维生素 E 的水平接近正常化。此外，百里香酚的体外还原能力研究证实了其强大的抗氧化作用。因此，百里香酚通过抗脂质过氧化和抗氧化作用来保护 ISO 诱导的大鼠心肌梗死。
