Department of Environmental and Occupational Health Sciences, University of Washington, Seattle, WA.
Department of Environmental and Occupational Health Sciences, University of Washington, Seattle, WA.
Am J Infect Control. 2011 Oct;39(8):628-632. doi: 10.1016/j.ajic.2010.11.007.
Methicillin-resistant Staphylococcus aureus (MRSA) isolated from frequently touched dental school clinic surfaces were compared with MRSA isolated nasal cultures of dental students.
Sixty-one dental students and 95 environmental surfaces from 7 clinics were sampled using SANICULT (Starplex Scientific Inc, Etobicoke, Ontario, Canada) swabs. Antimicrobial susceptibility testing was performed, and pulsed-field gel electrophoresis analysis, the mecA gene, multilocus sequence type, and SCCmec type were determined by polymerase chain reaction and sequencing.
Thirteen (21%) dental students and 8 (8.4%) surfaces were MRSA positive. Three MRSA strains were SCCmec type IV, whereas 3 were nontypeable isolates and Panton-Valentine leukocidin positive (PVL+), and none were USA300. One surface and 1 student isolate shared the same multilocus sequence type ST 8 and were 75% related. Two groups of students carried the same MRSA strains.
The MRSA-positive samples were from 4 of 7 dental clinics. In addition, 21% of the dental students carried MRSA, which is > 10 times higher than the general public and twice as frequent as in other university students. This is the first study to characterize MRSA from dental clinic surfaces and dental students and suggests that both may be reservoirs for MRSA. Further studies are needed to verify this premise.
从经常接触的牙科诊所表面分离出的耐甲氧西林金黄色葡萄球菌（MRSA）与牙科学生的鼻腔培养物分离出的 MRSA 进行了比较。
使用 SANICULT（Starplex Scientific Inc，安大略省多伦多市）拭子对 61 名牙科学生和 7 个诊所的 95 个环境表面进行采样。进行了抗菌药物敏感性测试，并通过聚合酶链反应和测序确定了脉冲场凝胶电泳分析、mecA 基因、多位点序列型和 SCCmec 型。
13 名（21%）牙科学生和 8 名（8.4%）表面为 MRSA 阳性。3 株 MRSA 为 SCCmec 型 IV，3 株为不可分型分离株且对 Panton-Valentine 白细胞毒素（PVL+）呈阳性，均非 USA300 型。1 个表面和 1 个学生分离株具有相同的多位点序列型 ST8，相似度为 75%。两组学生携带相同的 MRSA 株。
MRSA 阳性样本来自 7 个牙科诊所中的 4 个。此外，21%的牙科学生携带 MRSA，这是普通人群的 10 倍以上，是其他大学生的两倍。这是首次对牙科诊所表面和牙科学生的 MRSA 进行特征描述的研究，表明两者都可能是 MRSA 的储库。需要进一步的研究来验证这一前提。
