School of Marine and Tropical Biology and AIMS@JCU, Australian Institute of Marine Science and School of Marine and Tropical Biology, James Cook University, Townsville, Queensland 4811, Australia.
Mar Pollut Bull. 2012;65(4-9):384-93. doi: 10.1016/j.marpolbul.2011.08.020. Epub 2011 Sep 29.
Tropical coastal ecosystems, including the Great Barrier Reef (GBR) of Australia are increasingly threatened by pollution; yet few studies have investigated the sensitivity of GBR species to these pollutants. Here we exposed juveniles of the tropical reef fish Acanthochromis polyacanthus (spiny damselfish) to three concentrations of the insecticide chlorpyrifos (CPF) and measured (i) muscle cholinesterase (ChE) activity; (ii) hepatic glutathione-S-transferase (GST) activity; and (iii) coenzyme Q (CoQ) redox balance, after 6h and 96h of exposure. After 96h, muscle ChE activity was significantly inhibited by 26%, 49% and 53% when fish were exposed to 1, 10 or 100μg/L CPF, respectively. Muscle ChE characterization revealed three types of ChEs, including two atypical forms. Hepatic CoQ antioxidant form significantly increased at 10μg/L after 6h of exposure, potentially demonstrating an early response to CPF-induced oxidative stress in liver. Hepatic GST was not affected by CPF exposure.
热带沿海生态系统，包括澳大利亚的大堡礁（GBR），越来越受到污染的威胁；然而，很少有研究调查 GBR 物种对这些污染物的敏感性。在这里，我们将热带珊瑚鱼 Acanthochromis polyacanthus（棘尾鱼）的幼鱼暴露于三种浓度的杀虫剂毒死蜱（CPF）中，并在暴露 6 小时和 96 小时后测量了（i）肌肉胆碱酯酶（ChE）活性；（ii）肝谷胱甘肽-S-转移酶（GST）活性；以及（iii）辅酶 Q（CoQ）氧化还原平衡。96 小时后，当鱼暴露于 1、10 或 100μg/L CPF 时，肌肉 ChE 活性分别被显著抑制了 26%、49%和 53%。肌肉 ChE 的特征表明存在三种类型的 ChE，包括两种非典型形式。暴露于 CPF 6 小时后，肝 CoQ 抗氧化形式显著增加，可能表明肝脏对 CPF 诱导的氧化应激有早期反应。肝 GST 不受 CPF 暴露的影响。
