Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi, 214122, China.
Appl Microbiol Biotechnol. 2015 Feb;99(3):1109-18. doi: 10.1007/s00253-014-6298-y. Epub 2014 Dec 31.
Metabolic engineering facilitates the rational development of recombinant bacterial strains for metabolite overproduction. Building on enormous advances in system biology and synthetic biology, novel strategies have been established for multivariate optimization of metabolic networks in ensemble, spatial, and dynamic manners such as modular pathway engineering, compartmentalization metabolic engineering, and metabolic engineering guided by genome-scale metabolic models, in vitro reconstitution, and systems and synthetic biology. Herein, we summarize recent advances in novel metabolic engineering strategies. Combined with advancing kinetic models and synthetic biology tools, more efficient new strategies for improving cellular properties can be established and applied for industrially important biochemical production.
代谢工程促进了用于代谢产物过量生产的重组菌株的合理开发。在系统生物学和合成生物学的巨大进步的基础上，已经建立了用于在集合、空间和动态方式下对代谢网络进行多变量优化的新策略，例如模块化途径工程、隔室化代谢工程以及基于基因组尺度代谢模型、体外重构以及系统和合成生物学的代谢工程指导。在此，我们总结了新型代谢工程策略的最新进展。结合推进的动力学模型和合成生物学工具，可以建立更有效的用于改善细胞特性的新策略，并将其应用于工业上重要的生化生产。
