Rosenblatt J D, Zack J A, Chen I S, Lee H
Department of Medicine, UCLA School of Medicine.
Nat Immun Cell Growth Regul. 1990;9(3):143-9.
The human T-cell leukemia viruses type I (HTLV-I) and type II (HTLV-II) have been implicated in the pathogenesis of a variety of neoplastic and neurological disorders. Classical techniques for detection involve assay of serum for antibodies by Western blotting or ELISA, which do not discriminate between infection with HTLV-I and HTLV-II. In order to provide appropriate prognostic information to infected individuals and to obtain an accurate assessment of the prevalence of both retroviruses in the United States, we and others have applied the technique of enzymatic DNA amplification to detect HTLV-I and HTLV-II. These techniques allow rapid detection of viral nucleic acids in freshly isolated peripheral blood samples. Recent studies indicate an unusually high rate of HTLV-II infection among seropositive individuals in a sampling of New Orleans intravenous drug users, indicating a need for combined serological and molecular genetic screening of high-risk populations.
人类I型T细胞白血病病毒（HTLV-I）和II型T细胞白血病病毒（HTLV-II）与多种肿瘤性疾病和神经疾病的发病机制有关。传统的检测技术包括通过蛋白质免疫印迹法或酶联免疫吸附测定法检测血清中的抗体，这些方法无法区分HTLV-I感染和HTLV-II感染。为了向受感染个体提供适当的预后信息，并准确评估这两种逆转录病毒在美国的流行情况，我们和其他人应用了酶促DNA扩增技术来检测HTLV-I和HTLV-II。这些技术能够快速检测新鲜分离的外周血样本中的病毒核酸。最近的研究表明，在新奥尔良静脉注射吸毒者的抽样调查中，血清反应阳性个体中HTLV-II感染率异常高，这表明有必要对高危人群进行血清学和分子遗传学联合筛查。
