Faculty of Chemistry, Jagiellonian University, Krakow, Poland.
Analyst. 2012 Sep 21;137(18):4135-9. doi: 10.1039/c2an35331h. Epub 2012 Aug 2.
Attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) with the use of a slide-on germanium accessory followed by chemometric analysis allowed for providing meaningful information about the biochemical composition of a single endothelial cell. In this work, the methodology of the ATR-FTIR measurements of dried cells and dried cells immersed in water solution is presented. The contact of the cell and Ge crystal was set up manually and monitored through the integration of the amide I band. Additionally, the cell imaging in transreflection mode was tested, but the spectral differences between sub-cellular structures were not prominent in the registered spectra. It has been shown that the ATR-FTIR method gives better results due to the increased spatial resolution and S/R ratio as well as small contribution of the optical artifacts.
衰减全反射傅里叶变换红外光谱（ATR-FTIR）结合滑入式锗配件的使用，再加上化学计量学分析，可提供有关单个内皮细胞生化组成的有意义信息。在这项工作中，介绍了干燥细胞和干燥细胞浸入水溶液的 ATR-FTIR 测量方法。通过酰胺 I 带的积分来手动设置细胞和 Ge 晶体的接触，并对其进行监测。此外，还测试了反透射模式下的细胞成像，但在记录的光谱中，亚细胞结构之间的光谱差异并不明显。结果表明，ATR-FTIR 方法由于空间分辨率和 S/R 比值的提高以及光学伪影的贡献较小，因此结果更好。
