马都拉牛基因多态性（单核苷酸多态性c.73T>C）及其与体重和体尺的关联
Polymorphism of gene (single nucleotide polymorphisms c.73T>C) and its association with body weight and body measurements in Madura cattle.
作者信息
Kuswati Kuswati, Furqon Ahmad, Septian Wike Andre, Susilawati Trinil
机构信息
Department of Animal Production, Faculty of Animal Science, Universitas Brawijaya Jl. Veteran, Malang 65145, Indonesia.
出版信息
Vet World. 2022 Mar;15(3):775-781. doi: 10.14202/vetworld.2022.775-781. Epub 2022 Mar 30.
BACKGROUND AND AIM
Madura cattle is local cattle in Indonesia. This cattle hasphenotypic variations in growth traits. This study aimed to identify (LEP) gene polymorphism exon 2 associated with body measurements in Madura cattle.
MATERIALS AND METHODS
We recorded body weight (BW) and body measurements of 51 Madura cattle aged 1-4 years in Waru District, Madura. The LEP gene genotyping was conducted using the polymerase chain reaction-restricted fragment length polymorphism method with I restriction enzyme.
RESULTS
A 267 bp DNA fragment of the LEP gene was successfully amplified using a pair of primers. This study revealed three genotypes (TT, TC, and CC) and two alleles (T and C). The frequencies of TT, TC, and CC genotypes were 0.275, 0.45, and 0.275, respectively, whereas the frequencies of T and C alleles were 0.500 and 0.500, respectively. The c.73T>C mutation was significantly associated with BW, body length (BL), and chest girth (CG) (p0.05). Among all genotypes, the TC had the highest BW, BL, and CG.
CONCLUSION
Conclusively, LEP gene polymorphism (c.73T>C) exon 2 was polymorphic and associated with body measurements, especially BW, BL, and CG.
背景与目的
马都拉牛是印度尼西亚的本土牛种。这种牛在生长性状上存在表型变异。本研究旨在鉴定马都拉牛中与体尺相关的瘦素（LEP）基因外显子2的多态性。
材料与方法
我们记录了马都拉瓦鲁区51头1至4岁马都拉牛的体重（BW）和体尺数据。采用聚合酶链反应-限制性片段长度多态性方法，用I型限制性内切酶对LEP基因进行基因分型。
结果
使用一对引物成功扩增出LEP基因的一段267 bp的DNA片段。本研究揭示了三种基因型（TT、TC和CC）和两个等位基因（T和C）。TT、TC和CC基因型的频率分别为0.275、0.45和0.275，而T和C等位基因的频率分别为0.500和0.500。c.73T>C突变与BW、体长（BL）和胸围（CG）显著相关（p0.05）。在所有基因型中，TC的BW、BL和CG最高。
结论
总之，LEP基因外显子2的多态性（c.73T>C）具有多态性，且与体尺相关，尤其是BW、BL和CG。
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