Schaeffer B K, Zurlo J, Longnecker D S
Department of Pathology, Dartmouth Medical School, Hanover, New Hampshire 03756.
Mol Carcinog. 1990;3(3):165-70. doi: 10.1002/mc.2940030310.
The objective of this study was to determine whether activation of c-Ki-ras occurs in carcinogen-induced rat pancreatic tumors. DNAs from 27 samples, which included adenomas, carcinomas in situ, and adenocarcinomas arising in azaserine-treated rats and corn oil-gavaged rats along with a nafenopin-induced rat pancreatic adenocarcinoma were examined for mutation of c-Ki-ras at codons 12, 13, and 61 by using the polymerase chain reaction. Our results indicate that activation of c-Ki-ras is not a common event during pancreatic carcinogenesis in the rat.
本研究的目的是确定在致癌物诱导的大鼠胰腺肿瘤中是否发生了c-Ki-ras的激活。使用聚合酶链反应检测了27个样本的DNA，这些样本包括用氮杂丝氨酸处理的大鼠和经玉米油灌胃的大鼠产生的腺瘤、原位癌和腺癌，以及一种萘芬诺平诱导的大鼠胰腺腺癌，检测其c-Ki-ras第12、13和61密码子的突变情况。我们的结果表明，c-Ki-ras的激活在大鼠胰腺癌发生过程中并非常见事件。
