Longnecker D S, Pettengill O S, Davis B H, Schaeffer B K, Zurlo J, Hong H L, Kuhlmann E T
Department of Pathology, Dartmouth Medical School, Hanover, New Hampshire 03756.
Am J Pathol. 1991 Feb;138(2):333-40.
Nodules of acinar cells with increased proliferative potential develop in the pancreas of carcinogen-treated rats and in untreated aged rats. Large nodules are classed as adenomas. Phenotypic and genotypic characteristics of nodule cells were compared with normal pancreas and transplantable acinar cell carcinomas by several methods. Nuclei of acinar cells from normal pancreas, adenomas, and three carcinomas in situ had normal diploid DNA content as determined by flow cytometry. One of two primary carcinomas had a hypodiploid DNA content. Two of three transplantable carcinomas were aneuploid with a DNA content in the tetraploid range. Explants from nodules and adenomas failed to grow in soft agar, whereas several carcinomas were positive in this assay. A primary carcinoma was serially transplanted, but transplantation of nodules or adenomas failed. Transfection of DNA from carcinomas in situ yielded a higher frequency of NIH 3T3 transformants than DNA from adenomas. DNAs from the transformants did not contain ras sequences. These studies indicate that cells from nodules and adenomas have low growth potential and lack critical phenotypic and genotypic characteristics of transformed malignant cells that were present in some primary and transplanted carcinomas.
在经致癌物处理的大鼠胰腺以及未经处理的老龄大鼠胰腺中，会出现具有增殖潜能增强的腺泡细胞结节。大的结节被归类为腺瘤。通过多种方法，将结节细胞的表型和基因型特征与正常胰腺以及可移植性腺泡细胞癌进行了比较。通过流式细胞术测定，正常胰腺、腺瘤以及三个原位癌的腺泡细胞核具有正常的二倍体DNA含量。两个原发性癌中的一个具有亚二倍体DNA含量。三个可移植癌中的两个为非整倍体，DNA含量在四倍体范围内。来自结节和腺瘤的外植体在软琼脂中无法生长，而几种癌在该试验中呈阳性。一个原发性癌被连续传代移植，但结节或腺瘤的移植失败。来自原位癌的DNA转染产生的NIH 3T3转化体频率高于来自腺瘤的DNA。转化体的DNA不包含ras序列。这些研究表明，结节和腺瘤中的细胞生长潜能较低，并且缺乏一些原发性和移植性癌中存在的转化恶性细胞的关键表型和基因型特征。
